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Table 1 Short-term vs. Long-term effects of lack of PKA on induction of different genes by glucose

From: Transcriptional responses to glucose in Saccharomyces cerevisiae strains lacking a functional protein kinase A

Strain Growth conditions Gene
   PDC1 PDC5 ALD5 TMT1 YMC2 ATR1
   Induction factor
TPK1 TPK2 TPK3 30 min with glucose 12.8 235 5 9.7 11 13.8
  growth in medium with glucose 27 1.3 3 2 4 2.3
tpk1 tpk2 tpk3 msn2 msn4 30 min with glucose 15 70 3.4 1.7 2.2 2.6
  growth in medium with glucose 21 4.3 3.2 1.9 1.9 2.9
tpk1 tpk2 tpk3 yak1 30 min with glucose 1.2 1.8 0.9 1.1 0.8 1.4
  growth in medium with glucose 4.4 2 0.6 0.7 0.7 0.9
  1. For short-term experiments yeasts were grown on ethanol as described in Methods and samples were taken before the addition of glucose to the medium and 30 min after. The yeasts were also grown on a medium containing 2% ethanol and 4% glucose and collected when over 50% of the glucose was still present in the medium. Biological duplicates were performed and mRNA levels were measured by RT-PCR as described. Expression data from biological replicates generally differed from the average by less than 25%. The induction factor is the quotient of the values measured in samples grown in the presence of glucose and in the absence of glucose.