Transcriptional responses to glucose in Saccharomyces cerevisiae strains lacking a functional protein kinase A

BMC Genomics

Table 3 Genes with different expression in strains tpk1 tpk2 tpk3 msn2 msn4 and tpk1 tpk2 tpk3 yak1 during growth on a gluconeogenic carbon source

Gene	WT	tpk1 tpk2 tpk3 msn2 msn4	tpk1 tpk2 tpk3 yak1
HXK1*	459	77	228
HSP104*	327	121	229
MSC1*	629	58	296
SOL4*	148	28	107
YKL187c	321	28	124
GLC3*	390	96	425
GLK1*	852	465	889
GPX1*	271	41	196
GSY2*	184	45	245
HSP12*	3790	807	2436
HPF1*	650	137	611
NCA3	744	134	683
SOD1*	1964	841	1558
STF2	838	183	1004
TPS1*	569	245	612
TSL1*	274	45	245
ATF2	31	33	173
PRM5	116	339	164
SSA2	997	1519	611
DAL3	198	403	58
FSH1	220	271	68

Yeast strains TPK1 TPK2 TPK3, tpk1 tpk2 tpk3 msn2 msn4, and tpk1 tpk2 tpk3 yak1, were grown on ethanol and samples were taken before the addition of glucose to the medium, as described in Methods. mRNA levels were measured using Affymetrix microarrays. Biological duplicates were performed. Expression data from biological replicates generally differed from the average by less than 20%. Expression is shown in arbitrary units. (* Genes known to be activated by Msn2/4).

ISSN: 1471-2164