Histological and transcriptome-wide level characteristics of fetal myofiber hyperplasia during the second half of gestation in Texel and Ujumqin sheep

Table 8 Validation of differential expression by quantitative PCR (qPCR)

		Ratio of gene expression between two sheep breeds¹
Gene	Method	T70/U70	T85/U85	T100/U100	T120/U120	T135/U135
EEF1A2	qPCR	0.23	1.36	4.17	0.57	0.11
	microarray	8.92	16.21*	1.78	0.78	0.98
TXNRD1	qPCR	131.73	382.54	35.06	2.3	1.16
	microarray	1.38	1.14	5.20*	3.15	0.94
INSIG1	qPCR	0.82	1.65	0.32	0.16	0.10
	microarray	1.10	1.11	0.34*	0.88	0.87
LEF-1	qPCR	6.64	21.65	0.51	0.78	0.16
	microarray	0.52	0.59	0.47*	0.50	0.57
TMOD4	qPCR	81.5	714.32	323.03	3.7	0.02
	microarray	2.80*	3.52*	1.54	1.15	0.80
TPM2	qPCR	0.29	0.87	1.48	0.17	0.84
	microarray	0.48*	0.60	1.54	3.03	1.10
MYO9A	qPCR	0.14	0.15	0.25	0.31	0.12
	microarray	0.49*	0.61	1.02	0.75	1.20
TRRAP	qPCR	0.06	0.30	0.85	1.11	0.42
	microarray	0.51	0.35	1.79	0.20*	0.26
TNC	qPCR	0.21	0.54	5.12	6.23	10.46
	microarray	0.88	0.69	0.41*	0.62	1.78
PIK3R1	qPCR	0.61	0.44	0.23	1.06	0.29
	microarray	0.51	0.36*	1.03	1.92	1.14
ACTB	qPCR	1.79	0.16	0.73	0.35	0.85
	microarray	1.09	0.55	0.48*	1.39	0.47
MYL6B	qPCR	0.35	1.0	3.44	0.84	0.85
	microarray	0.42*	1.14	0.88	1.16	1.04
MYH1	qPCR	0.84	1.36	0.21	0.57	0.56
	microarray	1.89	5.60*	1.56	1.19	1.09
CASQ1	qPCR	0.51	0.76	0.56	0.07	2.79
	microarray	2.16 *	2.31	1.23	0.82	0.72
MSTN	qPCR	0.81	0.71	0.54	0.47	0.61
	microarray	0.69	0.92	0.62	0.56	0.81

1. The data in the rows are the ratio of the 2^ΔΔCT values for the qPCR in a Texel sheep sample relative to that in the Ujumqin sheep sample at the same developmental stage. The microarray row provides gene expression ratios in Texel sheep samples divided by those in Ujumqin sheep samples at the same developmental stage. *Differentially expressed genes in the microarray analysis with a statistically significant difference at p < 0.05.

ISSN: 1471-2164