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Table 1 Microdissection and RNA yield

From: Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection

Follicular stage Compartment Replicates Estimation of the number of sections input aRNA yield (μg)
Primordial Oocyte 3 130-160 7-28
  Granulosa 3 600 6-49
Primary Oocyte 4 35-100 3-28
  Granulosa 4 100-490 2-24
Secondary Oocyte 4 10-22 2-13
  Granulosa 4 Corresponding to 12-20 follicles 4-16
Small antral Oocyte 2 13-15 4-20
  Granulosa 2 Corresponding to 12-13 follicles 44-55
  1. Estimated number of microdissection sections input and corresponding yield of aRNA for each sample.
  2. aRNA: amplified RNA