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Table 1 Microdissection and RNA yield

From: Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection

Follicular stage

Compartment

Replicates

Estimation of the number of sections input

aRNA yield (μg)

Primordial

Oocyte

3

130-160

7-28

 

Granulosa

3

600

6-49

Primary

Oocyte

4

35-100

3-28

 

Granulosa

4

100-490

2-24

Secondary

Oocyte

4

10-22

2-13

 

Granulosa

4

Corresponding to 12-20 follicles

4-16

Small antral

Oocyte

2

13-15

4-20

 

Granulosa

2

Corresponding to 12-13 follicles

44-55

  1. Estimated number of microdissection sections input and corresponding yield of aRNA for each sample.
  2. aRNA: amplified RNA