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Table 2 Results of qPCR analysis

From: Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection

Gene Microarray analysis    qPCR analysis   
   Compartment effect (O/GC) Follicular stage Interactions  
  O/GC
Fold change
Fold change P value class Fold change P value class Compartment/stage Expression profile
Oocyte () GC ()
SOHLH2
VASA
WEE
O only 194.5
184.6
2.2
***
***
*
   *
MAEL
MATER
GDF9
BMP15
SIRT7
2.3
1.5
1.3
5.2
229.4
14.3
374.5
316.7
33.0
***
*
***
***
**
4.2
33.9
13.9
627.2
12.2
*
***
*
*
***
*
***
**
*
***
KL
GATA 4
LAS1L
PHGDH
-7.5
-2.1
GC only
GC only
-3.6
-8.7
-1.8
-3.6
**
*
*
NS: 0.067
   
AMH
FST
-7.3 -54.7
-697.5
*
*
1608.8
122.3
**
*
**
*
  1. O/GC fold change corresponds to the highest oocyte expression of the 3 follicular stages versus the lowest ones. For genes overexpressed in GC, a minus sign was added to the GC/O fold change.
  2. Follicular stage fold change corresponds to the highest expression stage versus the lowest within a compartment.
  3. For each gene, a one-way ANOVA model was fitted with the 2 factors: "stage" (4 levels) and "compartment" (2 levels) with interactions using R statistical software.
  4. The expression profile column visualizes the expression level of each compartment: bold line is used for oocyte expression and double line for GC expression. The slope symbolizes a significant increase of the expression during the follicular development.
  5. P-value class: *: p < 0.05; **: p < 0.01; ***:p < 0.001; NS: non specific
  6. Abbreviations: O: oocyte (red line), GC: granulosa cells (blue line)