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Table 2 Results of qPCR analysis

From: Transcriptome profiling of sheep granulosa cells and oocytes during early follicular development obtained by Laser Capture Microdissection

Gene

Microarray analysis

  

qPCR analysis

  
  

Compartment effect (O/GC)

Follicular stage

Interactions

 
 

O/GC

Fold change

Fold change

P value class

Fold change

P value class

Compartment/stage

Expression profile

Oocyte () GC ()

SOHLH2

VASA

WEE

O only

194.5

184.6

2.2

***

***

*

  

*

MAEL

MATER

GDF9

BMP15

SIRT7

2.3

1.5

1.3

5.2

229.4

14.3

374.5

316.7

33.0

***

*

***

***

**

4.2

33.9

13.9

627.2

12.2

*

***

*

*

***

*

***

**

*

***

KL

GATA 4

LAS1L

PHGDH

-7.5

-2.1

GC only

GC only

-3.6

-8.7

-1.8

-3.6

**

*

*

NS: 0.067

   

AMH

FST

-7.3

-54.7

-697.5

*

*

1608.8

122.3

**

*

**

*

  1. O/GC fold change corresponds to the highest oocyte expression of the 3 follicular stages versus the lowest ones. For genes overexpressed in GC, a minus sign was added to the GC/O fold change.
  2. Follicular stage fold change corresponds to the highest expression stage versus the lowest within a compartment.
  3. For each gene, a one-way ANOVA model was fitted with the 2 factors: "stage" (4 levels) and "compartment" (2 levels) with interactions using R statistical software.
  4. The expression profile column visualizes the expression level of each compartment: bold line is used for oocyte expression and double line for GC expression. The slope symbolizes a significant increase of the expression during the follicular development.
  5. P-value class: *: p < 0.05; **: p < 0.01; ***:p < 0.001; NS: non specific
  6. Abbreviations: O: oocyte (red line), GC: granulosa cells (blue line)