Distribution of clade specific SNPs, Typhi-associated virulence genes and β- glucuronidase activity among 169 S. enterica subsp. enterica isolates representing 98 serovars. Clade specific SNPs and the presence of Typhi associated virulence genes were determined using SNP assays, TaqMan® assays or full genome sequence data. β- glucuronidase activity was determined using a phenotypic assay. If multiple isolates representing the same serovar had the same characteristics the number of isolates is indicated by n = in parenthesis, ** indicates that multiple isolates of the same serovar had different characteristics. Serovars colored blue have three clade B specific SNPs, light green indicates serovars with two out of three clade B specific SNPs, yellow indicates serovars which are β-glucuronidase positive and have one out of three clade B specific SNPs, purple indicates serovars which are β-glucuronidase negative and have one out of three clade B specific SNPs, red indicates serovars that have three clade A specific SNPs and tested positive for SPI-18 and the CdtB-islet, and orange indicates serovars that have three clade A specific SNPs and that tested positive for one or two toxin related genes.