Real-time qRT-PCR validation of microarray data analysis on the three major organs. (A) Gene expression fold changes of six target genes as determined by microarray and real-time qRT-PCR experiments. The direction and magnitude of fold changes obtained from the real-time qRT-PCR technique were comparable to those obtained from the microarray technique. # P < 0.05 for gene expression fold changes quantified by real-time PCR experiments as determined by two-tailed unpaired Student's t-test. (B) Correlation of gene expression fold changes between microarray and real-time qRT-PCR data. Validation of the microarray data via real-time qRT-PCR shows that correlation of fold changes obtained by these two gene expression profiling techniques was high with an R2 = 0.9653.