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Table 2 Genes selected for the real-time qRT-PCR validation experiments

From: Differential transcriptomic profiles effected by oil palm phenolics indicate novel health outcomes

Organ Symbol Definition Accession Assay ID
Liver Cyp3a11 Mus musculus cytochrome P450, family 3, subfamily a, polypeptide 11 NM_007818 Mm00731567_m1
Liver Hmgcs1 Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 NM_145942 Mm00524111_m1
Spleen Vwf Mus musculus Von Willebrand factor homologue NM_011708 Mm00550376_m1
Spleen Brca1 Mus musculus breast cancer 1 NM_009764 Mm00515386_m1
Heart Pdk4 Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 NM_013743 Mm00443325_m1
Heart Rad21 Mus musculus RAD21 homologue (S. pombe) NM_009009 Mm00485474_m1
All Sfrs9 Mus musculus splicing factor, arginine/serine rich 9 NM_025573 Mm00470546_m1
All Guk1 Mus musculus guanylate kinase 1 NM_008193 Mm00433888_m1
All Hnrpab Mus musculus heterogeneous nuclear ribonucleoprotein A/B NM_010448 Mm00468938_m1
  1. These six target genes were selected based on their presence in significant functions, their differential scores, their detection levels, their presence as single splice transcripts in microarrays and their availability as Taqman assays designed across splice junctions. Sfrs9, Guk1 and Hnrpab were used as housekeeping genes as their expression levels were found to be quite stable across treatments in each of the organs tested.