Differential transcriptomic profiles effected by oil palm phenolics indicate novel health outcomes

BMC Genomics

Table 2 Genes selected for the real-time qRT-PCR validation experiments

Organ	Symbol	Definition	Accession	Assay ID
Liver	Cyp3a11	Mus musculus cytochrome P450, family 3, subfamily a, polypeptide 11	NM_007818	Mm00731567_m1
Liver	Hmgcs1	Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1	NM_145942	Mm00524111_m1
Spleen	Vwf	Mus musculus Von Willebrand factor homologue	NM_011708	Mm00550376_m1
Spleen	Brca1	Mus musculus breast cancer 1	NM_009764	Mm00515386_m1
Heart	Pdk4	Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4	NM_013743	Mm00443325_m1
Heart	Rad21	Mus musculus RAD21 homologue (S. pombe)	NM_009009	Mm00485474_m1
All	Sfrs9	Mus musculus splicing factor, arginine/serine rich 9	NM_025573	Mm00470546_m1
All	Guk1	Mus musculus guanylate kinase 1	NM_008193	Mm00433888_m1
All	Hnrpab	Mus musculus heterogeneous nuclear ribonucleoprotein A/B	NM_010448	Mm00468938_m1

These six target genes were selected based on their presence in significant functions, their differential scores, their detection levels, their presence as single splice transcripts in microarrays and their availability as Taqman assays designed across splice junctions. Sfrs9, Guk1 and Hnrpab were used as housekeeping genes as their expression levels were found to be quite stable across treatments in each of the organs tested.

ISSN: 1471-2164