Table 4 Transcriptomic analysis of P.aeruginosa M18 at 28°C and 37°C

Up-regulated gene at 28°C

Prophage I

PAM18_1347, PAM18_1349-1350, PAM18_1352-1355

1.8-3.4

MGI II

hsdR, hsdM

1.8-2.4

MGI III

pltR, pltZ, pltHIJNO

1.4-2.7

MGI V

PAM18_5670-PAM18_5675

2.8-5.4

Phage related

PAM18_0614, PAM18_0616-617, PAM18_0619, PAM18_0620, PAM18_0622-0625, PAM18_0626, PAM18_0627

2.3-8.4

TCA cycle

PAM18_1548-PAM1550

2.1-2.2

Oxidative phosphorylation

cyoC, cyoA, napE, napF, napD, napABC

1.5-2.7

FeS cluster assembly

iscS, iscU, iscA

1.8-2.2

Glyoxylate metabolism

fdnI, fdnH, fdnG

1.6-1.6

Benzonate degradation

amiE, PAM18_1600, amiC, amiR, PAM18_1603, bkdB, bkdA1, bkdA2

1.7-4.5

Phosphate uptake

PAM18_1584, phnE, PAM18_1586, phnJ, phoQ, phoP

1.6-2.7

Copper resistance

pcoA, pcoB

1.4-1.5

Pentose phosphate

rbsK, rbsR, rbsA, rbsB

1.6-1.9

Regulatory proteins

rhlI, qscR, PAM18_0792, PAM18_0975, PAM18_3016, PAM18_0807, PAM18_3383

2.0-6.1

Down-regulated genes at 28°C

Type III secretion system

exoT, exsD, PAM18_0335, exsC, popD, popB, pcrH, pcrV

2.1-2.8

Type II secretion system

xcpP, xcpR, xcpT, secY

1.6-2.7

Protease production and secretion

lasA, aprA, aprD, aprE, aprF, pfpI, PAM18_0458

2.3-11.4

HCN synthesis

hcnA, hcnB, hcnC

1.5-2.9

Phenazine metabolism and transport

phzA1-G1, phzM, phzA2-G2, opmD, mexI, mexH

1.6-11.6

Fimbrial biogenesis

cupC1, pilA, pilN

2.2-3.0

AHL biosynthesis

lasI

2.5

Phospholipase C

plcB

3.3

Detoxification

PAM18_1269, PAM18_1457, PAM18_1515, PAM18_4192

2.2-4.6

Benzonate degradation

PAM18_0222-0224, PAM18_0250, phhABC, phhR, aroP2, hpd, hmgA, antABC, catABC

2.1-57.0

Transportation

PAM18_0605-0606, PAM18_1782-1783, PAM18_1785, PAM18_2831-2832, PAM18_3039, PAM18_3041-3043, PAM18_5348-5350, PAM18_4688, PAM18_1003

2.0-13.3

Sulfur metabolism

PAM18_3206-3207, PAM18_2369-2372

2.1-3.4

Iron uptake

PAM18_4792-4793, PAM18_4818-4819, PAM18_3537, PAM18_3606, PAM18_4448, PAM18_4605

Amino acid metabolism

liuA, liuD, liuR, PAM18_3044-3046, arcABC

Heat shock proteins

ibpA, dnaK, groEL

2.1-3.5

Energy production and conversion

nqrC, nqrE, nuoEGHIJLMN, atpABCDFGH, nirM, nirS, sucABCD, lpdG, PAM18_3490-3491

1.6-4.1

Ribosomal proteins

rplABCDEFJKLMNOPQRSWX, rpmCDEHJ, rpsCEFGHJKLMNQRS

1.5-3.8

Regulatory proteins

PAM18_0249, PAM18_0710, PAM18_0945, PAM18_0966, PAM18_1156, PAM18_1507, PAM18_2031, PAM18_2078, PAM18_2114, PAM18_2280, PAM18_2571, PAM18_2608, PAM18_3035, PAM18_3129, PAM18_3270, PAM18_3440, PAM18_4328, PAM18_5219, PAM18_5273

2.0-3.9