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Table 2 Cisplatin sensitivity of Ub-proteasome pathway deletion mutants

From: Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast

Gene ID Annotation IC50 (mM) ± SD Cisplatin response
Mutant strainsa    
SPAC26A3.16 dph1 0.068 ± 0.023  
SPAC328.06 ubp2 0.079 ± 0.06  
SPAC24H6.03 pcu3 0.053 ± 0.006  
SPAC167.07c cul3 0.048 ± 0.03  
SPAC12B10.01c UB-lig (E3) 0.133 ± 0.06 R
SPBC6B1.06c ucp2, ubp14 0.08 ± 0.02  
SPCC1682.12c ubp16 0.0098 ± 0.0002 HS
SPBP8B7.21 ubp3 0.11 ± 0.08  
SPBC19C7.02 ubr1 0.153 ± 0.01 R
SPBP8B7.27 mug30 0.149 ± 0.001 R
SPCC1442.07c UB metalloprotease 0.061 ± 0.006  
SPAC27F1.03c uch1 0.066 ± 0.018  
SPAC31G5.18c UB family... 0.025 ± 0.05  
SPCC188.08c ubp22/ubp15 0.065 ± 0.011  
SPAC11E3.04c ubc13/spu13 0.0175 ± 0.01 HS
SPAC10F6.07c ubc6 0.062 ± 0.011  
SPAC1250.03 ubc14 0.069 ± 0.012  
SPAC13A11.04c ubp8 0.044 ± 0.003  
SPBC800.12c UB family... 0.057 ± 0.006  
SPBC106.16 pre6 0.053 ± 0.01  
SPBC409.06 uch2 0.052 ± 0.02  
SPBC530.03c bag102/bag1-b 0.193 ± 0.023 R
SPBC16G5.03 UB-lig (E3) 0.051 ± 0.013  
SPBC19G7.09 ulp1(SUMO-deconj) 0.054 ± 0.016  
SPCC1919.15 brl1 0.052 ± 0.019  
SPBC2A9.04c UB-lig (E3) 0.196 ± 0.005 R
SPBC577.10 20S (sub.β7) 0.170 ± 0.011 R
SPAC17G8.10c dma1 0.063 ± 0.004  
SPAC6G10.11c ubi3 0.188 ± 0.004 R
SPAC20H4.10 ufd2 0.17 ± 0.0006 R
SPBC19C2.04c ubp11 0.171 ± 0.006 R
SPBC15C4.06c UB-lig (E3) 0.055 ± 0.006  
SPAC23G3.08c ubp7 0.06 ± 0.004  
SPBC23G7.12c let1/rpt6 (19S) 0.18 ± 0.007 R
SPBC887.04c lub1 0.054 ± 0.005  
SPAC15A10.11 ubr11 0.062 ± 0.019  
SPAC1198.09 ubc16 0.032 ± 0.013  
SPAC17C9.13c cut8 0.028 ± 0.002  
SPBC18H10.08c ubp4 0.054 ± 0.019  
SPCC790.02 pep3 0.024 ± 0.003  
SPBC16E9.11c pub3 0.059 ± 0.005  
SPAC1805.15c pub2 0.062 ± 0.005  
SPCC1682.16 rpt4 0.03 ± 0.02  
Wild-type straina    
ED668: h+ ade6-M216/ura4-D18/leul-32   0.09 ± 0.01  
Mutant strainsa    
SPAC6G9.08 ubp6 0.052 ± 0.008  
SPBC6B1.05c atg7 0.073 ± 0.009  
SPBC2D10.20 ubc1 0.055 ± 0.015  
SPBC365.06 pmt3 0.0096 ± 0.003 HS
Wild-type straina    
ED666: h+ ade6-M210/ura4-D18/leul-32   0.045 ± 0.02  
Cisplatin sensitive straina
SPBC216.05 rad3 0.01 ± 0.009  
  1. a Sensitivity was evaluated using a microtiter growth inhibition assay with a 48 h drug exposure. IC50 values are the means ± standard deviation of at least three independent experiments. The IC50 was defined as the drug concentration that reduced the absorbance to 50% of the value measured for the untreated control culture, and was compared to cisplatin sensitivity of the corresponding wild-type parental strain. Hypersensitive strains are indicated as HS and resistant strains as R.
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