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Table 2 Cisplatin sensitivity of Ub-proteasome pathway deletion mutants

From: Ubiquitin-proteasome genes as targets for modulation of cisplatin sensitivity in fission yeast

Gene ID

Annotation

IC50 (mM) ± SD

Cisplatin response

Mutant strainsa

   

SPAC26A3.16

dph1

0.068 ± 0.023

 

SPAC328.06

ubp2

0.079 ± 0.06

 

SPAC24H6.03

pcu3

0.053 ± 0.006

 

SPAC167.07c

cul3

0.048 ± 0.03

 

SPAC12B10.01c

UB-lig (E3)

0.133 ± 0.06

R

SPBC6B1.06c

ucp2, ubp14

0.08 ± 0.02

 

SPCC1682.12c

ubp16

0.0098 ± 0.0002

HS

SPBP8B7.21

ubp3

0.11 ± 0.08

 

SPBC19C7.02

ubr1

0.153 ± 0.01

R

SPBP8B7.27

mug30

0.149 ± 0.001

R

SPCC1442.07c

UB metalloprotease

0.061 ± 0.006

 

SPAC27F1.03c

uch1

0.066 ± 0.018

 

SPAC31G5.18c

UB family...

0.025 ± 0.05

 

SPCC188.08c

ubp22/ubp15

0.065 ± 0.011

 

SPAC11E3.04c

ubc13/spu13

0.0175 ± 0.01

HS

SPAC10F6.07c

ubc6

0.062 ± 0.011

 

SPAC1250.03

ubc14

0.069 ± 0.012

 

SPAC13A11.04c

ubp8

0.044 ± 0.003

 

SPBC800.12c

UB family...

0.057 ± 0.006

 

SPBC106.16

pre6

0.053 ± 0.01

 

SPBC409.06

uch2

0.052 ± 0.02

 

SPBC530.03c

bag102/bag1-b

0.193 ± 0.023

R

SPBC16G5.03

UB-lig (E3)

0.051 ± 0.013

 

SPBC19G7.09

ulp1(SUMO-deconj)

0.054 ± 0.016

 

SPCC1919.15

brl1

0.052 ± 0.019

 

SPBC2A9.04c

UB-lig (E3)

0.196 ± 0.005

R

SPBC577.10

20S (sub.β7)

0.170 ± 0.011

R

SPAC17G8.10c

dma1

0.063 ± 0.004

 

SPAC6G10.11c

ubi3

0.188 ± 0.004

R

SPAC20H4.10

ufd2

0.17 ± 0.0006

R

SPBC19C2.04c

ubp11

0.171 ± 0.006

R

SPBC15C4.06c

UB-lig (E3)

0.055 ± 0.006

 

SPAC23G3.08c

ubp7

0.06 ± 0.004

 

SPBC23G7.12c

let1/rpt6 (19S)

0.18 ± 0.007

R

SPBC887.04c

lub1

0.054 ± 0.005

 

SPAC15A10.11

ubr11

0.062 ± 0.019

 

SPAC1198.09

ubc16

0.032 ± 0.013

 

SPAC17C9.13c

cut8

0.028 ± 0.002

 

SPBC18H10.08c

ubp4

0.054 ± 0.019

 

SPCC790.02

pep3

0.024 ± 0.003

 

SPBC16E9.11c

pub3

0.059 ± 0.005

 

SPAC1805.15c

pub2

0.062 ± 0.005

 

SPCC1682.16

rpt4

0.03 ± 0.02

 

Wild-type straina

   

ED668: h+ ade6-M216/ura4-D18/leul-32

 

0.09 ± 0.01

 

Mutant strainsa

   

SPAC6G9.08

ubp6

0.052 ± 0.008

 

SPBC6B1.05c

atg7

0.073 ± 0.009

 

SPBC2D10.20

ubc1

0.055 ± 0.015

 

SPBC365.06

pmt3

0.0096 ± 0.003

HS

Wild-type straina

   

ED666: h+ ade6-M210/ura4-D18/leul-32

 

0.045 ± 0.02

 

Cisplatin sensitive straina

SPBC216.05

rad3

0.01 ± 0.009

 
  1. a Sensitivity was evaluated using a microtiter growth inhibition assay with a 48 h drug exposure. IC50 values are the means ± standard deviation of at least three independent experiments. The IC50 was defined as the drug concentration that reduced the absorbance to 50% of the value measured for the untreated control culture, and was compared to cisplatin sensitivity of the corresponding wild-type parental strain. Hypersensitive strains are indicated as HS and resistant strains as R.
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BMC Genomics

ISSN: 1471-2164

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