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Figure 4 | BMC Genomics

Figure 4

From: Comprehensive SNP array study of frequently used neuroblastoma cell lines; copy neutral loss of heterozygosity is common in the cell lines but uncommon in primary tumors

Figure 4

Clonal differences between cell lines. A) Genome-wide chromatograms for the highly related cell lines; SK-N-SH, SH-EP and SH-SY5Y. Aberrations shared by all three cell lines are shown as black arrows, those that are shared by two of the cell lines are shown as grey arrows and the ones unique to each cell line are shown as unfilled arrows. Note that all three cell lines have acquired new aberrations since the sub cloning took place. The gains seen at 1 q for SH-SY5Y and SH-EP, although similar in this picture, are not identical in position and copy number and probably represent two different events. Only aberrations larger than 1.5 Mb are indicated in the figure, with the exception of the homozygous deletion at 9p21 in SH-EP. B) MLPA results confirming the deletion at 9p21 found in SH-EP, as well as the small gain found in SH-SY5Y in this region. Neither of these aberrations can be seen in the parental cell line SK-N-SH. The whole chromosome gain at chromosome 7, seen in all three cell lines, is also confirmed by the MLPA analysis.

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