A combined functional and structural genomics approach identified an EST-SSR marker with complete linkage to the Ligon lintless-2 genetic locus in cotton (Gossypium hirsutum L.)

Table 2 Expression ratios and statistical significances from the microarray and RT-qPCR data

Microarray		DOA (WT/Mutant)		8 DPA (WT/Mutant)		12 DPA (WT/Mutant)
Probesets ID	Blastx Sequence Description	Microarray	RT-qPCR	Microarray	RT-qPCR	Microarray	RT-qPCR
Ghi.2036.1.S1_s_at	Cu/Zn superoxide dismutase (GhCSD1)	1.48*	1.80*	2.52*	5.22*	1.78*	4.72*
Ghi.1711.1.S1_s_at	Putative SANT/MYB Transcription Factor	0.80*	0.51*	0.19*	0.65	0.16*	0.34*
Ghi.3235.1.A1_at	Putative UDP-glycosyltransferase (UGT73C2)	0.72*	0.53	0.03*	0.0077*	0.23*	0.033*
Ghi.4377.1.A1_at	Glycuronosyltransferase-like protein	0.78*	0.67	3.83*	7.85*	1.20*	2.67*
Ghi.6551.1.S1_at	BZIP domain class transcription factor	0.72*	0.57*	1.90*	2.59*	2.94*	1.84*
Ghi.7279.1.S1_at	Putative ABC transporter	1.43*	1.22*	3.82*	6.86*	6.03*	5.44*
Ghi.7450.1.S1_s_at	ECERIFERUM 3 (CER3)	0.79*	0.81	2.31*	2.35*	3.17*	1.64*
Ghi.7724.1.S1_at	Cellulose-synthase-like C5 (AtCSLC5)	0.98*	0.67*	2.44*	5.96*	4.37*	3.97*
Ghi.8115.1.S1_s_at	Transcription factor GhMYB109	0.97*	0.71*	5.02*	3.45*	7.80*	4.67*
Ghi.8665.1.S1_s_at	Sucrose synthase	1.17	1.05	0.72	0.53	1.11	0.64
Ghi.9209.1.S1_at	Putative R2R3-Myb transcription factor	0.94*	1.30	0.27	0.60	0.53	1.00
Ghi.9236.1.S1_at	Putative UDP-glycosyltransferase (UGT73C5/DOGT1)	0.92*	0.25*	0.08*	0.0035*	0.55	0.0083*
Gra.2056.1.A1_s_at	Beta-galactosidase	0.41*	0.13	39.90*	101.53*	41.57*	38.62*

* Microarray data: significant at the Bonferroni-corrected 0.05 probability level; RT-qPCR data: significant at the 0.05 probability
level as determined by two-tailed paired t-test for means. Microarray and RT-qPCR data significant at ≥ 2-fold difference in transcript abundance are shown in boldface and underlined.

ISSN: 1471-2164