Table 5 Primers used for qRT-PCR analyses to validate deep sequencing data
Gene | Forward primer | Reverse Primer |
|---|---|---|
Storage protein 1 | CAAGACACGCTACGACGC | GTCGGCATGACGAAGTAC |
Insulin receptor | GTACCCCTCGATCTCGCG | CCCACGTCAAGGGAACCC |
JH epoxide hydrolase | AGGATCTACGCGGAGGGC | TGGTACACCACTTCGTAC |
Ecdysteroid regulated protein | AACCCGAAGAGCCGAAGC | CTCTGTAGTCGCTGCTAC |
Cuticle protein | CAGGATGACGAGTCTGGC | GTCTGCCTCGTATTCTAC |
Toll receptor | CCTCCGGCAACGCCCTAG | CGCACAGAAATTCAGAGG |
Hemolin | AGCTCCAGAGACTACGCC | GTGTTGTAGGAACCATTG |
Gloverin | AGCTAGCCCGGCATCCGC | GACGGTAGCCCGCCTTAC |
pxSerpin 3 | GAATAGCTTCTACTACGC | TGATAGCGAATTCGGTAC |
Actin | ATGGAGAAGATCTGGCAC | GGAGCCTCCGTGAGCAGC |