Table 5 Characterization of 40 EST-SSRs among 24 sesame accessions
Primer | SSRs | Forward primer (5'-3') | Reverse primer (5'-3') | No. of alleles | Ho | He | PIC |
|---|---|---|---|---|---|---|---|
ZM_1 | (CA)6 | GTTTCTTGGTCTTATCACAGC | TACCAACGTCACTCTTCTTTC | 5 | 1.00 | 0.72 | 0.66 |
ZM_2 | (AC)8 | CTTCTTGAAGTTCTGGTGTTG | ATTCTTGGAGAAAGAGTGAGG | 6 | 0.91 | 0.77 | 0.72 |
ZM_3 | (GT)8 | ATCACCACACACTGACACAG | CGTGTCTGAGAATCCAATATC | 7 | 0.96 | 0.79 | 0.74 |
ZM_4 | (AC)8 | TCCAGAGAGGAGACAATAAGA | GAGATAGATTGCGAGTTGTGT | 4 | 0.71 | 0.72 | 0.64 |
ZM_5 | (CT)10 | GATAAAGAACTGCCAAGGAAC | CACAGCAGTGAAGAAAAGAGT | 6 | 0.71 | 0.74 | 0.68 |
ZM_6 | (GT)9 | GGTGTGTTCTCTCTCTCACAC | GGGCTGCTCAATAAATGTAG | 6 | 0.75 | 0.78 | 0.73 |
ZM_7 | (AGC)6 | ATCCTCTGCTCCTAACTTCAT | TCTGGTACTATCCTCAAGCAA | 6 | 0.96 | 0.79 | 0.74 |
ZM_8 | (AG)6 | TCTCTCTCTCTCTCGTTCTTG | CCCACTGTACCTCTCCATATT | 5 | 0.70 | 0.73 | 0.66 |
ZM_9 | (AAT)5 | CTCGCCGTTCTAACATTATC | AGTACAGTCTCCTCCGATTTT | 5 | 0.50 | 0.72 | 0.65 |
ZM_10 | (CTTCCT)4 | ATGCCCATCTCCATATACTCT | AATTCTTGCCTGACTCTACG | 8 | 0.83 | 0.81 | 0.76 |
ZM_11 | (CT)12 | GGATTCTCTAGACATGGCTTT | AACGCAGAATTCTCTCCTACT | 8 | 1.00 | 0.85 | 0.81 |
ZM_12 | (CT)7 | ATTGCTGTGCAATCCTTATC | ATCTCTTTCTACCACCACGTT | 5 | 1.00 | 0.79 | 0.74 |
ZM_13 | (GA)7 | GCAGAAGGCAATAAAGTCAT | GCGTCAGAAGAAAAATACTGG | 7 | 1.00 | 0.80 | 0.75 |
ZM_14 | (ATC)5 | GGAAGGCGAGTTGATAGATAA | CATGGGATGTTCAAAGAACT | 6 | 0.96 | 0.79 | 0.73 |
ZM_15 | (GA)7 | ACTCTATCACCGAGTGGAGAC | CTACCCTTTTCCTCGTAGC | 7 | 0.65 | 0.75 | 0.69 |
ZM_16 | (GATC)4 | AGGTAGAATTACATGCTGTGC | GCTTCCTCCTTCATTCATATC | 5 | 0.60 | 0.71 | 0.65 |
ZM_17 | (CT)6 | CTTGCTTCCTCTTTTCTCTCT | ACACTGTACTCAGCGGATTT | 5 | 1.00 | 0.79 | 0.72 |
ZM_18 | (CT)6 | AATACCCTTCAGTATTCAGGTG | CAACAACACAAACACTGCTAC | 5 | 1.00 | 0.80 | 0.75 |
ZM_20 | (GAG)5 | GGGATGTTGATAGAGATGTTG | TCTTTCACTCTCACACACACA | 10 | 1.00 | 0.86 | 0.82 |
ZM_21 | (AC)7 | CTCTCTCTCTCTGCTGTTTCA | GCCATACGATCTCAAAATCAC | 8 | 0.95 | 0.86 | 0.82 |
ZM_22 | (AT)9(GA)6 | ACCACCGATCTACTCACTTTT | CCACTGCACACTACAGTTTTT | 9 | 0.68 | 0.86 | 0.82 |
ZM_23 | (AT)7(GA)8 | CGTATGTCAAGATGAAGCAGT | ATCAACAATTCCACTCAACC | 6 | 0.95 | 0.75 | 0.69 |
ZM_24 | (TC)6(CCATTT)4 | CCACACTCAAAACCAAGAAA | GCGAAGAGATTATATACACACG | 5 | 0.75 | 0.72 | 0.66 |
ZM_25 | (AT)6 | CCTGAACCTTCTCTCTCTCTC | ACTGACAGTACGAATTCACCA | 4 | 0.58 | 0.71 | 0.64 |
ZM_26 | (GA)8 | ACTTCAACTTCAACCTCAACC | TGTGCATAAAAACCCTCTCT | 5 | 1.00 | 0.65 | 0.57 |
ZM_29 | (AG)9 | CATTACAATAGCCCGAAAAG | TACTGTTCCTCCTCCTCTCTT | 6 | 1.00 | 0.70 | 0.62 |
ZM_30 | (TAT)5 | CACTCCACTCATTATCCAAAG | CAAGACACAACTGACACGTAA | 6 | 1.00 | 0.77 | 0.72 |
ZM_31 | (GA)7 | GAGCACTCTCTTCTCTCTTCC | AAAAGAGGATGGCAACTGTA | 5 | 0.78 | 0.72 | 0.65 |
ZM_32 | (GCC)5 | CACGAAGAGTGAGAGAGAGAG | CTACCAAAAGTCCCTGAATCT | 5 | 0.77 | 0.70 | 0.62 |
ZM_33 | (TCA)5 | GAGACAGTACACTTGGGACAA | CTCTTCTTGGGCATTAACTCT | 6 | 0.74 | 0.73 | 0.67 |
ZM_34 | (AG)14 | AAGTCCCTTTTCAAGCAATC | GAGAGAGGAAAATGCAGAGAG | 10 | 0.79 | 0.83 | 0.79 |
ZM_35 | (TTCC)4 | AATGCATAGTGCATAGGGTAG | TGGAAAGTAGAGATCGCATAG | 6 | 0.96 | 0.75 | 0.69 |
ZM_38 | (GA)8 | CAGCTTCCTGATTTGATTTG | AGATTGCAAGAATCGCTTAG | 5 | 0.85 | 0.69 | 0.62 |
ZM_39 | (TCA)5 | AGAGGCAGAGGAGTTGATAAT | CTTAACTGTAACTCCCTTTTCG | 6 | 0.81 | 0.78 | 0.72 |
ZM_40 | (ACTCCA)5 | CGAAAAGGGAGTTACAGTTAAG | CTTCCTCTCCTATCATCCTGT | 7 | 0.82 | 0.82 | 0.77 |
ZM_41 | (AC)8 | GATATGATTCAAACCCCTCAG | CTTCTGCACTACCATCAATTC | 5 | 0.63 | 0.55 | 0.46 |
ZM_43 | (ACAT)4 | CTTGGATATCAGTTTCCTGTG | GTTCTCCACAGTCAAAACACT | 7 | 0.89 | 0.72 | 0.65 |
ZM_44 | (TC)9 | GTCTTAAGCCCTCTTAGTTCC | GAAAACCTTCAATGTCAGGA | 7 | 0.77 | 0.77 | 0.73 |
ZM_45 | (TA)6 | GCAAAATCTCTGTTGTCTCAG | GTGTTCCTACCACTCAACACA | 18 | 0.83 | 0.83 | 0.80 |
ZM_47 | (TC)8 | GTTTCCAGGTCTATTCCTTTG | AGGTAGAGCTAATCCTTACCG | 10 | 0.71 | 0.83 | 0.79 |
Mean | Â | Â | Â | 6.55 | 0.84 | 0.76 | 0.70 |