Characteristics of homology search of assembled sequences against the nr database. A. E-value distribution of best BLASTx hits for each distinct sequence with a cut-off E-value of 1.0E-5. B. Similarity distribution of the best BLAST hit for each sequence. C. Species distribution is shown as a percentage of the total homologous sequences with an E-value of at least 1.0E-5. We used the first hit of each sequence for analysis. Homo: Homo sapiens; Mus: Mus musculus; Rat: Rattus norvegicus.