Confirmation of microarray results by qPCR. Expression levels of six genes were compared, as listed in Additional file 4. Log2-ER from microarrays was plotted on the y-axis, while log2-transformed fold changes from real-time qPCR were plotted on the x-axis. The four replicate pools of heart tissue from 4 wpi against control pools were measured for each gene. The dashed black line represents the regression function of the measured values. The function is shown in the top left corner of the plot. In addition, the Pearson correlation and the corresponding p-value are shown.