Figure 4

The generation of short by-products is conserved across species. Analysis of distances between 5' ends of overlapping sequences from opposite chromosome strands from rat (A) and platypus (B) testes lysates reveals the processing patterns that were already observed in mouse. (C) In mouse testes lysate treated with beta-elimination, 18-mer sequences contribute mostly to the P28 peak, suggesting that the by-products are not 3' methylated. (D) The histogram for the long (23-32 nt) and short (15-22 nt) sequences that contribute to the P28 pattern.