Figure 4

Control of adipocyte differentiation by FACS studies. The adipocytic differentiation was also monitored using flow cytometry with different markers. After growing 14 days some of the cells were harvested and marked with the monoclonal antibodies CD26, CD29, CD49b, CD55, CD73, CD90, CD140a (all Becton Dickinson, San Jose, CA), CD49f, CD120a (both Serotec, Raleigh, NC) and CD105 (Caltag, Buckingham, UK). As a negative control, cells were incubated with the corresponding irrelevant mouse IgG1, IgG2a, IgG2b or IgGM mAbs - therefore, cells became visible in the background. But only if a gene expression is changing in relation to the background after 14 days, can the result be positive. In Figure 4 the cells were colored early in the beginning with a blue marker and after 14 days with a red coloration. Only mAb CD140a clearly showed an increased gene expression after 14 days.