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Table 1 Sequence Mapping Statistics

From: Whole-exome sequencing of DNA from peripheral blood mononuclear cells (PBMC) and EBV-transformed lymphocytes from the same donor

Sample Total reads Primer Mapped reads per primer % of reads mapped Total sequence per primer (Gb) Total sequence (Gb) Uniquely mapped reads % of reads uniquely mapped Uniquely mapped sequence (Gb) Total uniquely mapped sequence (Gb) Mean Coverage % of targets with ≥ 8× coverage
ND02537_LCL 92248252 F3
F5
74121470
61566483
80.4%
66.7%
3.70
2.15
5.86 59773418
49605343
80.6%
80.6%
2.98
1.73
4.72 124.34 91.02%
ND02537_PBMC 93437335 F3
F5
73787463
61874203
79.0%
66.2%
3.68
2.16
5.85 59060679
49242442
80.0%
79.6%
2.95
1.72
4.67 123.07 90.34%
ND02538_LCL 83047764 F3
F5
66056191
56555527
79.5%
68.1%
3.30
1.97
5.28 53355146
45327323
80.8%
80.1%
2.66
1.58
4.25 111.95 91.20%
ND02538_PBMC 94548773 F3
F5
74721895
65.5%
79.0%
0.655
3.73
2.16
5.90 59775898
48973298
80.0%
79.1%
2.98
1.71
4.70 123.76 90.11%
ND02539_LCL 99014161 F3
F5
76834989
65785009
77.6%
66.4%
3.84
2.30
6.14 61133857
50648022
79.6%
77.0%
3.05
1.77
4.82 127.09 89.91%
ND02539_PBMC 98836019 F3
F5
81875758
68523012
82.8%
69.3%
4.09
2.39
  66054927
54336918
80.7%
79.3%
3.30
1.90
5.20 136.96 90.21%
ND02540_LCL 93619368 F3
F5
77310874
65926759
82.6%
70.4%
3.86
2.30
6.17 63328774
52941514
81.9%
80.3%
3.16
1.85
5.01 132.09 90.12%
ND02540_PBMC 97278109 F3
F5
77375008
62335812
79.5%
64.1%
3.86
2.18
6.05 61792168
49552780
79.9%
79.5%
3.08
1.73
4.82 126.95 91.22%
Average 94003723 F3
F5
75260456
63059668
80.1%
67.1%
3.76
2.20
5.97 60534359
50078455
80.4%
79.4%
3.02
1.75
4.77 125.78 90.52%
  1. Total reads: total number of sequence reads generated for the sample; Primer F3: 50 bp sequence; Primer F5: 35 bp sequence; Total sequence per primer: number of Gb of sequence generated for each primer; Uniquely mapped reads: number of reads which mapped only once to the genome; Total uniquely mapped reads: total Gb of uniquely mapped sequence generated; Mean coverage: average number of times each uniquely mapped base-pairs was sequenced