Figure 2

Correspondence between MBP1 motif binding, nucleosome depletion and expression pattern. A. Comparison of nucleosome occupancy between MBP1 bound motifs (blue line) [29] and unbound (black line) consensus sites CGCGT[CT] over the S. cerevisiae intergenic region. Comparison of nucleosome signals from -300 to 300 reveals that overall the bound (B) motifs (arrow) are more nucleosome-depleted than unbound (C) motifs although exceptions occur. D. Comparison of expression patterns during the cell cycle for genes with at least one bound MBP1 motif (blue line, as assessed by chromatin immuno-precipitation) in the region upstream of the start site, and genes (black line) that have an MBP1 consensus-match in the upstream intergenic region but that are not bound by the MBF protein complex. The cyclic expression patterns of genes with bound Mbp1p (E) are more apparent than the genes with an MBP1 motif present but without evidence of binding (F).