Figure 3

Unsupervised hierarchical clustering of the gene expression profiles. (A) Classification of the 33 biological samples using unsupervised hierarchical clustering of the gene expression profiles (31,918 contigs) employing Pearson's correlation. Numbers along the branches indicate bootstrap values after 100 iterations. Labial palp and gill tissue samples from the same individuals grouped together (1Pm and 1Bm; 5Pf and 5Bf; 6Pf and 6Bf). (B) Heat map of selected genes specifically overexpressed in different tissue types. Individual columns represent individual biological samples. The number of genes enriched in each tissue is indicated in Table 1. (C) Heat map of selected genes specifically over-expressed in two tissue types. (D) Expression of annotated and non-annotated genes potentially involved in regulating food-intake and energy balance. The cluster of genes was obtained using hierarchical clustering with Pearson's correlation on the tissue-enriched genes. For each biological sample, numbers identify the individuals (individuals 1 to 8) and letters show the pools of 6 individuals (a, b, c and d), upper letters identify the tissues (G: Gonad; GI: Gills; P: Labial palps; M: Mantle; MS: Adductor muscle; DG: Digestive gland; GG: visceral ganglia; HE: Hemocytes), followed by m for males (individuals 1, 7 and 8) and f for females (individuals 2, 3, 5 and 6).