Table 3 Ranking of 3 commonly used reference genes for quantitative real time PCR experiments on oysters (spot: location on microarray, ID genbank: accession number, stdev: standard deviation, description: best BLAST hit description as described in GigasDatabase, CV: coefficient of variation, max: maximum value, min: minimum value, MFC: maximum fold change, p value: ANOVA 1 factor, P > 0.05, NR: Not Ranked)
RANK | spot | ID genbank | description (GigasDatabase) | MEAN | stdev | CV | max | min | MFC | p value |
|---|---|---|---|---|---|---|---|---|---|---|
256 | 11936 | BG467400 | (sp:Q9YIC0) Elongation factor 1-alpha OS = Oryzias latipes GN = eef1a PE = 2 SV = 1 | 4.08 | 0.10 | 0.03 | 4.29 | 3.86 | 1.11 | 0.41 |
NR | 10575 | AJ544886 | (sp:P56649) Glyceraldehyde-3-phosphate dehydrogenase OS = Panulirus versicolor PE = 1 SV = 1 | 6.17 | 0.20 | 0.03 | 6.57 | 5.79 | 1.13 | < 0.05 |
NR | 26871 | AF026063 | (sp:O17320) Actin OS = Crassostrea gigas PE = 2 SV = 1 | 6.41 | 0.16 | 0.02 | 6.67 | 6.01 | 1.11 | < 0.05 |