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Table 3 Quantitative PCR validation

From: Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

Gene

Contrast

qPCR

Microarray

AvBD 2

NV-C Severe Day 5 vs. NV-C Mild Day 5

-3.035*

-2.038**

 

NV-C Severe Day 1 vs. NV-NC Control Day 1

2.954**

1.552**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

-3.206**

-1.863**

 

NV-C Severe Day 1 vs. NV-C Severe Day 5

5.525**

2.931**

AvBD 6

NV-C Severe Day 1 vs. NV-NC Control Day 1

2.592**

1.719**

 

NV-C Severe Day 1 vs. NV-C Severe Day 5

4.726**

2.194**

AvBD 7

NV-C Severe Day 5 vs. NV-C Mild Day 5

-4.259

-3.440**

 

NV-C Severe Day 1 vs. NV-NC Control Day 1

1.214

1.731**

 

NV-C Severe Day 1 vs. NV-C Severe Day 5

4.446*

3.346**

IL1B

NV-C Mild Day 1 vs. NV-NC Control Day 1

0.567

3.826**

 

NV-C Severe Day 1 vs. NV-NC Control Day 1

1.290

1.836**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

2.296

1.960**

IL6

NV-C Severe Day 5 vs. NV-C Mild Day 5

2.263

1.117**

 

NV-C Severe Day 1 vs. NV-NC Control Day 1

3.027*

1.227**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

2.611

1.138**

IFNG

NV-C Severe Day 5 vs. NV-C Mild Day 5

2.399**

1.154**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

2.427**

2.000**

TLR 2

NV-C Severe Day 1 vs. NV-NC Control Day 1

-0.070

1.140**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

0.699

0.984**

TLR 4

NV-C Severe Day 5 vs. NV-NC Control Day 5

1.396*

1.336**

MD-2

NV-C Severe Day 5 vs. NV-C Mild Day 5

0.028

5.311**

 

NV-C Severe Day 5 vs. NV-NC Control Day 5

0.034

1.230**

IL1-R, type II

NV-C Severe Day 1 vs. NV-C Mild Day 1

1.574 **

1.263**

 

NV-C Severe Day 1 vs. NV-NC Control Day 1

1.241

2.676**

  1. Log2 fold change between contrasts presented. + values indicate higher expression in the first group, - values indicate higher expression in the second group. ** P value < 0.05 in qPCR, q-value < 0.05 in microarray, * P value < 0.10 in qPCR