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Table 3 Quantitative PCR validation

From: Spleen transcriptome response to infection with avian pathogenic Escherichia coli in broiler chickens

Gene Contrast qPCR Microarray
AvBD 2 NV-C Severe Day 5 vs. NV-C Mild Day 5 -3.035* -2.038**
  NV-C Severe Day 1 vs. NV-NC Control Day 1 2.954** 1.552**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 -3.206** -1.863**
  NV-C Severe Day 1 vs. NV-C Severe Day 5 5.525** 2.931**
AvBD 6 NV-C Severe Day 1 vs. NV-NC Control Day 1 2.592** 1.719**
  NV-C Severe Day 1 vs. NV-C Severe Day 5 4.726** 2.194**
AvBD 7 NV-C Severe Day 5 vs. NV-C Mild Day 5 -4.259 -3.440**
  NV-C Severe Day 1 vs. NV-NC Control Day 1 1.214 1.731**
  NV-C Severe Day 1 vs. NV-C Severe Day 5 4.446* 3.346**
IL1B NV-C Mild Day 1 vs. NV-NC Control Day 1 0.567 3.826**
  NV-C Severe Day 1 vs. NV-NC Control Day 1 1.290 1.836**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 2.296 1.960**
IL6 NV-C Severe Day 5 vs. NV-C Mild Day 5 2.263 1.117**
  NV-C Severe Day 1 vs. NV-NC Control Day 1 3.027* 1.227**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 2.611 1.138**
IFNG NV-C Severe Day 5 vs. NV-C Mild Day 5 2.399** 1.154**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 2.427** 2.000**
TLR 2 NV-C Severe Day 1 vs. NV-NC Control Day 1 -0.070 1.140**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 0.699 0.984**
TLR 4 NV-C Severe Day 5 vs. NV-NC Control Day 5 1.396* 1.336**
MD-2 NV-C Severe Day 5 vs. NV-C Mild Day 5 0.028 5.311**
  NV-C Severe Day 5 vs. NV-NC Control Day 5 0.034 1.230**
IL1-R, type II NV-C Severe Day 1 vs. NV-C Mild Day 1 1.574 ** 1.263**
  NV-C Severe Day 1 vs. NV-NC Control Day 1 1.241 2.676**
  1. Log2 fold change between contrasts presented. + values indicate higher expression in the first group, - values indicate higher expression in the second group. ** P value < 0.05 in qPCR, q-value < 0.05 in microarray, * P value < 0.10 in qPCR