Figure 6

Expression patterns of six selected WRKY genes under abiotic stresses. In A-F, the top panel shows the RT-PCR result and the bottom panel shows the corresponding real-time PCR result. For real-time PCR, the relative amount of mRNA (y-axis) was calculated by according to the description in Methods. The cucumber β-actin gene was used as an internal control to normalize the data. The 0, 0.5, 1, 3, 6, 12, and 24 (x-axis) indicate the treatment time (hour) under corresponding abiotic stresses. The error bars were calculated based on three replicates. A-C, significant up-regulated expression of WRKY genes can be detected under abiotic stresses. D, significant down-regulated expression of CsWRKY53 can be detected under cold treatment. E, the expression difference detected by real-time PCR was more significant than that detected by RT-PCR. F, no significant expression difference can be detected in group 3 WRKY gene CsWRKY50 under abiotic stress. Statistical significance was obtained by using Student's t-test.