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Table 1 Strains and plasmids used in this study

From: Transcriptional responses of Burkholderia cenocepacia to polymyxin B in isogenic strains with diverse polymyxin B resistance phenotypes

Strain or Plasmid Description Source or Reference
Escherichia coli
DH5α F-, ϕ80 lacZ ΔM15 Δ(lacZYA-argF) U169 deoR endA1 recA1 hsdR17 (rK- mK+) thi-1 glnV44 Laboratory Stock
Burkholderia cenocepacia
K56-2 CF clinical isolate (polymxyin B-resistant) BCCRC*, [19]
RSF34 K56-2, ΔhldA (polymyxin B-sensitive) [18]
RSF34 25A RSF34 colony isolated on 25 μg/ml polymyxin B This study
RSF34 200E RSF34 25A colony isolated on 200 μg/ml polymyxin B This study
RSF34 1000D RSF34 200E colony isolated on 1 mg/ml polymyxin B This study
RSF34 4000B RSF34, selected through multiple rounds for polymyxin B resistance This study
RSF44 K56-2, ΔfliCD, flagella-negative [18]
Pseudomonas aeruginosa
PAO1 Non-CF clinical isolate [56]
Plasmids   
pRK2013 RK2 derivative, KmR mob+ tra+ oricolE1 [49]
pSL6 B. cenocepacia expression plasmid, rhamnose-inducible promoter, TpR CmR [9]
pSL7 pSL6, B. cenocepacia hldAD [9]
  1. *B. cepacia complex Research and Referral Repository for Canadian CF Clinics