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Table 2 Genes that respond similarly to 0.5 μM 20E, 10 μM 20E, or 0.0625 μM PoA

From: Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster

Gene Symbol or Clone ID

0.5 μM 20E Fold Difference

q value (%)

10 μM 20E Fold Difference

q value (%)

0.0625 μM PoA Fold Difference

q value (%)

GO Molecular Function

Eip71CD

4.07

0.00

3.58

0.00

3.51

0.00

oxidoreductase activity

CG5346

3.10

0.00

2.88

0.00

3.49

0.00

cation binding

Pect

2.88

0.00

2.91

0.00

2.49

0.00

transferase activity

PRL-1

2.10

0.00

2.12

0.00

1.58

0.00

hydrolase activity

CG4825

2.10

0.00

2.17

0.00

2.56

0.00

transferase activity

CG30104

2.03

0.00

2.40

0.00

2.39

 

hydrolase activity

eater

1.97

0.00

2.52

0.00

2.20

0.00

receptor binding

Cyp4e2

1.83

0.00

2.05

0.00

2.02

0.00

monooxygenase activity

CG14523

1.83

0.40

2.04

0.00

1.81

0.00

peptidase activity

granny-smith

1.79

0.00

1.79

0.00

1.94

0.00

cation binding

CG5958

1.53

0.40

1.65

0.00

1.82

0.00

retinal binding

RE28720

1.52

3.56

1.67

0.00

1.54

0.00

N/A

RH68619

1.49

0.00

1.75

0.00

1.68

0.00

N/A

mTerf3

1.46

4.45

1.69

0.00

1.70

0.00

protein binding

CG33969

1.40

0.84

1.50

0.00

1.19

0.73

N/A

Ggamma1

1.39

 

1.27

0.47

1.58

0.11

hydrolase activity

CG8507

1.38

 

1.18

4.43

1.57

0.00

low-density lipoprotein receptor binding

CG18591

1.35

 

1.26

0.47

1.62

0.16

RNA binding

HL03650

1.33

1.50

1.54

0.00

1.74

0.00

N/A

GstE1

1.05

 

-1.01

 

-1.52

0.00

transferase activity

RE45701

-1.22

2.76

-1.65

0.47

-1.56

0.00

N/A

CG7706

-1.22

 

-1.62

0.63

  

anion transporter activity

vas

-1.27

 

-1.52

0.00

  

ATP-dependent helicase activity

CG14739

-1.28

0.62

-1.53

0.63

  

ubiquitin conjugating enzyme activity

Pde8

-1.30

 

-1.49

0.00

-1.58

0.00

hydrolase activity

LD33681

-1.35

 

-1.31

0.00

-1.67

0.00

N/A

CG13868

-1.36

0.49

-1.35

0.30

-1.58

0.00

protein binding

Gs1

-1.39

2.17

-1.58

0.00

-1.07

 

ligase activity

arm

-1.39

3.56

-1.59

0.00

-1.30

0.14

alpha-catenin binding

Rpn1

-1.41

 

-1.15

 

-1.54

0.49

peptidase activity

CG10641

-1.47

0.00

-1.34

1.60

-1.58

0.00

cation binding

eIF3-S10

-1.55

0.00

-1.36

0.00

-1.75

0.28

translation factor activity

RhoGAP16F

-1.65

0.00

-1.34

0.00

-1.59

0.00

GTPase activator activity

RH07164

-1.86

4.45

-1.59

0.30

-1.86

0.14

N/A

  1. A multi-class SAM analysis revealed genes with similar transcriptional responses to the hormones and hormone concentrations studied. Genes identified as primary 20E-response genes (see Table 1) are indicated in bold. As with Table 1, the fold change and q-value of each gene following the treatment indicated is given. A missing fold change value means that gene was either not represented by at least 2/3 spots and/or did not exhibit a coefficient of variation of at least 1 for that treatment across replicate arrays.