Genome-wide examination of the transcriptional response to ecdysteroids 20-hydroxyecdysone and ponasterone A in Drosophila melanogaster

Table 4 Annotation enrichment of genes that respond to 20E in either Kc167 cells or salivary glands

Response to 20E	# Genes in Group with Valid Identifier/Total # in Group	Category	Term	p-value	Number in Category	% of Total
		SP	chromoprotein	3.3E-03	3	5
		INT	peptidase M13	4.3E-03	3	5
		BP	electron transport	4.5E-03	7	13
Induced in Kc cells	56/69	BP	metabolic process	5.5E-03	31	55
		MF	oxidoreductase activity	1.0E-02	10	18
		INT	glutathione S-transferase	1.0E-02	3	5
		BP	generation of precursor metabolites and energy	1.6E-02	7	13
		MF	iron ion binding	3.1E-02	5	9
		BP	cellularization	2.4E-04	6	11
		BP	salivary gland histolysis	4.9E-04	6	11
		BP	programmed cell death	2.8E-03	9	16
		BP	cellular developmental process	3.4E-03	18	32
		BP	exocrine system development	3.4E-03	6	11
		BP	germ-band shortening	5.7E-03	3	5
		BP	gland development	6.6E-03	6	11
		BP	sperm individualization	1.5E-02	3	5
Induced in salivary glands	87/98	UP	compositionally biased region:Poly-Ala	1.4E-02	5	9
		MF	kinase activity	1.4E-02	9	16
		BP	regulation of metabolic process	1.5E-02	15	27
		MF	ligand-dependent nuclear receptor activity	2.8E-02	3	5
		BP	cell differentiation	1.7E-02	16	29
		BP	female meiosis chromosome segregation	3.2E-02	3	5
		MF	cytoskeletal protein binding	3.0E-02	7	13
		BP	embryonic development via syncytial blastoderm	4.3E-02	5	9
		BP	regulation of cellular metabolic process	4.8E-02	13	23
		INT	cell division/GTP binding protein	5.2E-02	2	4
		SP	nucleotide-binding	3.2E-08	20	28
		MF	kinase activity	6.2E-04	11	15
Repressed in Kc cells	71/77	INT	chaperonin Cpn60	5.7E-03	3	4
		MF	unfolded protein binding	1.2E-02	4	6
		SP	glycolysis	1.5E-02	3	4
		BP	cytoskeleton organization and biogenesis	2.5E-02	9	13
Repressed in salivary glands	9	N/A	N/A	N/A	N/A	N/A

DAVID (http://david.abcc.ncifcrf.gov/) was used to identify enriched annotation categories among groups of genes that respond similarly to 20E. The modified Fisher Exact p-value represents the probability that the number of genes associated with an annotation term in the sample list is random compared to the occurrence of the term in the all of the genes on the array used for the analysis (12kv1)). (BP) biological process; (MF) molecular function; (CC) cellular component; (SP) Swiss Prot Keywords; (INT) Interpro Name; (UP) UniProt Sequence Feature.

ISSN: 1471-2164