Figure 2

The chromosomal inversion in CDC 684. Panel A: The att sites in two lambda-like prophages, Ba04 and Ba02. The att sites are illustrated as vertical bars and their positions define the insertion site for each prophage. The black vertical bars indicate the position of the external flanking edge of the att sites in each prophage. The black att sites are in the same relative positions in both the CDC 684 and Ames Ancestor genomes. However, the red and green att sites (bars) highlight the positions in the prophages that are inverted in the CDC 684 genome. The red and green arrows indicate putative sites where a homologous exchange caused the 3.3 Mbp inversion. Panel B describes the unique att sites and defines the size of each of the prophages. This panel also illustrates the translocation of the internal (red and green) att sites in CDC 684 to equivalent positions within the sister prophages, which are 3.3 Mbp apart. The new att sites for both Lambda Ba04 and Lambda Ba02 indicate that these two prophages in CDC 684 are now hybrid prophages containing unique 3' elements.