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Table 6 RT-qPCR validation of expression differences identified by transcriptome sequence analysis.

From: Sequence comparison of prefrontal cortical brain transcriptome from a tame and an aggressive silver fox (Vulpes vulpes)

    Transcriptome Sequencing Relative RT-qPCR
Accession Number Gene Fold difference between two samples Z statistics p value Number of reads in the sample of tame fox Number of reads in the sample of aggressive fox Sample ratio Fold difference between two samples Sample ratio
XM_848547 SCGN 2.24 6.28 3.28E-10 136 402 A>T 2.44 A>T
XM_544247 ITGA8 4.48 4.85 1.24E-06 68 20 T>A 5.55 T>A
XM_546414 CDON 5.95 3.51 4.44E-04 6 47 A>T 1.09 A>T
NM_001006648 HTR2C 9.22 2.69 0.01 14 2 T>A 2.89 T>A
NM_001003300 KCNMA1 2.19 2.65 0.01 26 75 A>T 1.43 A>T
XM_543946 LCOR 10.63 2.14 0.03 1 14 A>T 1.75 A>T
XM_849641 PRRG2 11.71 2.11 0.03 8 0 T>A 1.31 T>A
XM_846863 LRRC20 10.54 2.08 0.04 8 1 T>A 1.12 T>A
XM_542630 SLITRK6 9.87 2.04 0.04 1 13 A>T 1.54 T>A
  1. Nine genes, identified as differentially expressed between the tame and aggressive fox by transcriptome sequence analysis, were selected for expression evaluation by relative RT-qPCR. Results are sorted by Z test p-value. Except for one gene (SLITRK6, result in bold and italic), which had the least significant Z test p-value, RT-qPCR consistently confirmed a difference of expression between the tame and aggressive samples in the same direction as had transcriptome analysis (compare columns 8 and 10).