Effects of elevated seawater p CO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

BMC Genomics

Table 2 Enrichment analysis in the short-term hypercapnia microarray study on Carcinus maenas

Test-set	GO	name	FDR	Over-/Under- representation
down day 3	GO:0050794	regulation of cellular process	0.00	under
down day 3	GO:0007165	signal transduction	0.00	under
down day 3	GO:0023060	signal transmission	0.00	under
down day 3	GO:0023046	signaling process	0.00	under
down day 3	GO:0003824	catalytic activity	0.00	under
down day 3	GO:0050789	regulation of biological process	0.04	under
up day 3	no enrichment	-	-	-
down day 7	no enrichment	-	-	-
up day 7	no enrichment	-	-	-
up gill 7	GO:0007010	cytoskeleton organization	0.05	under
down gill 7	GO:0005623	cell	0.04	over
up gill 9	GO:0005198	structural molecule activity	0.03	over
down gill 9	no enrichment	-	-	-

Results of the enrichment analysis (Fisher's Exact Test with multiple testing correction; FDR < 0.05) as implemented in Blast2GO [43]. Tested were subsets of all significantly regulated transcripts as identified by sign test (up- and down-regulated separately for each gill and day or each combination of both). Reference-set in each analysis was the complete microarray sequence Blast2GO database generated from the contig list. GO = Gene Ontology term, name = GO name of the functional group, FDR = false discovery rate for correction of multiple testing, Over-/Underrepresentation = a GO term is considered over-/under-represented if it appears significantly more often/less often in the reference-set than it does in the test-set.

ISSN: 1471-2164