Effects of elevated seawater p CO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

Fehsenfeld, Sandra; Kiko, Rainer; Appelhans, Yasmin; Towle, David W; Zimmer, Martin; Melzner, Frank

doi:10.1186/1471-2164-12-488

BMC Genomics

Table 3 Regulation of transcripts of special interest in the short-term hypercapnia study on Carcinus maenas

From: Effects of elevated seawater p CO₂ on gene expression patterns in the gills of the green crab, Carcinus maenas

			CO₂ experiments 3 + 7 days				CO₂ experiments 11 weeks	Salinity experiments 7 days
			[MA; med. log2-ratio ± MD]				[qPCR; med. log2-ratio ± MD]	[MA; mean log2-ratio ± SD]
rank	ACC. no.	Name	gill 7 - day 3	gill 9 -day 3	gill 7 - day 7	gill 9 -day 7	gill 7 - 10 weeks	gill 8 - day 7
1	DV944570.1	senescence-associated protein	-0.12 ± 0.50	-0.03 ± 0.03	*-1.16 ± 0.64**	-0.07 ± 0.25	0.69 ± 0.57	-0.06 ± 0.03
2	DW250369.1	pg1 protein	1.01 ± 0.61	0.60 ± 1.14	-0.34 ± 0.49	-0.29 ± 0.56	-	1.644 ± 0.414
3	DN161290.1	unknown (putative Syntaxin binding protein 2)	0.32 ± 0.32	0.42 ± 0.10	*0.88 ± 0.10**	0.67 ± 0.31	0.49 ± 0.66	2.86 ± 0.02
4	DN796131.1	23S ribosomal RNA gene (a)	-1.20 ± 0.92	-0.25 ± 0.18	-1.14 ± 1.16	*-0.87 ± 0.46**	-	0.36 ± 0.34
5	DW584691.1	calcium-activated chloride channel	0.25 ± 0.40	*0.81 ± 0.34**	-0.09 ± 0.30	0.16 ± 0.31	1.12 ± 0.88	-
6	DN635040.1	23S ribosomal RNA gene (b)	-0.16 ± 1.42	0.09 ± 0.64	*-0.80 ± 0.22**	-0.27 ± 0.49	-	1.29 ± 0.16
7	DV944193.1	rRNA intron-encoded homing endonuclease	0.54 ± 1.00	0.80 ± 0.81	0.06 ± 0.22	0.59 ± 0.41	-	1.073 ± 0.38
8	DW249535.1	unknown	-0.12 ± 1.43	0.04 ± 0.55	*-0.71 ± 0.62**	-0.41 ± 0.65	-	0.99 ± 0.29
9	DY656451.1	unknown	-0.15 ± 0.23	0.05 ± 0.26	*0.57 ± 0.09**	0.04 ± 0.27	-	-0.15 ± 0.30
10	DW251049.1	unknown	-0.35 ± 0.24	*-0.53 ± 0.28**	-0.05 ± 0.22	-0.10 ± 0.19	-	-0.86 ± 0.23
11	DN551127.1	unknown	0.54 ± 0.25	0.26 ± 0.14	*0.48 ± 0.31**	0.37 ± 0.48	-	2.34 ± 0.07
12	DW250828.1	thymosin-repeated protein 1	-0.39 ± 0.21	*-0.46 ± 0.14**	0.13 ± 0.21	0.19 ± 0.21	-	-0.05 ± 0.06
13	DN202592.1	multispanning membrane protein (transmembrane 9 superfamily protein member 4)	0.35 ± 0.23	0.21 ± 0.14	*0.46 ± 0.11**	0.36 ± 0.34	-	2.15 ± 0.08
14	DW250260.1	hyperpolarization activated cyclic nucleotide-gated potassium channel-2	-0.26 ± 0.30	-0.14 ± 0.08	*-0.46 ± 0.24**	*-0.14 ± 0.08**	-0.13 ± 0.26	-0.11 ± 0.09
18	DV944030.1	tetraspanin 3 (Transmembrane super family 4 member 8)	-0.22 ± 0.11	*-0.41 ± 0.05**	0.01 ± 0.27	0.09 ± 0.03	-	-0.13 ± 0.06
25	DY307809.1	potential integrin alpha-7	-0.12 ± 0.36	0.26 ± 0.21	*-0.35 ± 0.28**	-0.03 ± 0.26	-	-
29	DV642643.1	kazal-type proteinase inhibitor	*0.25 ± 0.17**	*0.21 ± 0.12**	*0.33 ± 0.20**	*0.24 ± 0.15**	0.12 ± 0.84	1.04 ± 0.11
31	DN635395.1	unknown	*-0.17 ± 0.04**	*-0.14 ± 0.04**	*-0.32 ± 0.06**	*-0.13 ± 0.06**	0.25 ± 0.68	-0.11 ± 0.11
63	DV943872.1	predicted ATPase	-0.34 ± 0.33	-0.24 ± 0.10	-0.58 ± 0.39	*-0.23 ± 0.09**	-	-0.65 ± 0.23
88	CX994129.1	anion/bicarbonate transporter family member (SLC family 4, member 1)	-0.31 ± 0.11	*-0.20 ± 0.09**	-0.22 ± 0.07	-0.22 ± 0.11	0.18 ± 0.54	0.41 ± 0.11
-	DN739347.1	glycosyl-phosphatidylinositol-linked carbonic anhydrase VII	-0.09 ± 0.05	-0.06 ± 0.06	*-0.13 ± 0.07**	*-0.08 ± 0.03**	0.40 ± 0.35	-0.92 ± 0.07
-	DN551450.1	glutathione peroxidase	0.02 ± 0.06	0 ± 0.05	*0.03 ± 0.02**	-0.03 ± 0.05	-0.90 ± 0.24	-0.81 ± 0.14
-	DY656042.1	vacuolar-type H+-ATPase subunit A	-0.05 ± 0.37	0.11 ± 0.09	-0.11 ± 0.09	0.07 ± 0.06	0.45 ± 0.65	-0.17 ± 0.27
-	DY657461.1	v-type proton ATPase subunit E	0.02 ± 0.03	-0.02 ± 0.04	0 ± 0.03	-0.02 ± 0.03	0.30 ± 0.39	-0.12 ± 0.17
-	DW251095.1	cytoplasmic carbonic anhydrase	0.07 ± 0.01	-0.03 ± 0.06	0 ± 0.07	0.01 ± 0.01	-0.12 ± 1.20	1.78 ± 0.16
-	DN202373.1	anion/bicarbonate transporter family member (SLC family 4, member 11)	-0.02 ± 0.03	0.02 ± 0.06	-0.02 ± 0.10	0.01 ± 0.02	0.02 ± 0.64	-1.29 ± 0.47
-	DN796352.1	K+/Cl- symporter (solute carrier family 12, member 6)	-0.04 ± 0.04	0.22 ± 0.17	0.01 ± 0.04	-0.07 ± 0.07	0.91 ± 0.30	0.25 ± 0.23
-	DV111894.1	Na+/K+/2Cl- cotransporter	0.05 ± 0.05	0.01 ± 0.05	0 ± 0.06	0 ± 0.10	0.62 ± 0.57	-0.20 ± 0.09
-	DW250552.1	Na+/K+ ATPase alpha subunit	0.04 ± 0.05	0.02 ± 0.05	0.05 ± 0.10	0.03 ± 0.16	0 ± 1.11	1.38 ± 0.11
-	DY308103.1	Na+/H+ exchanger (NHE 3)	-	-	-	-	0.27 ± 0.03	-0.13 ± 0.14

Included are the 14 most strongly regulated transcripts (as identified by sign test and F-test), as well as several transcripts which have a potential function during structural modifications or in ion- and acid/base regulation. Transcripts are ordered according to the rank in absolute regulation. Accession numbers (ACC. no.) refer to the ESTs generated for the C. maenas microarray by Towle et al. [37] in the database GenBank (NCBI). Rank 1 = highest regulation; bold and underlined = significant regulation relative to the control identified by sign test; bold = identified to be differentially expressed by both tests; italic = identified to be differentially expressed only by F-test; "-" = a rank was not assigned in case the regulation was less than log₂-ratio = |0.20|.

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ISSN: 1471-2164

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