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Table 3 Regulation of transcripts of special interest in the short-term hypercapnia study on Carcinus maenas

From: Effects of elevated seawater p CO2 on gene expression patterns in the gills of the green crab, Carcinus maenas

    CO2 experiments 3 + 7 days CO2 experiments 11 weeks Salinity experiments 7 days
    [MA; med. log2-ratio ± MD] [qPCR; med. log2-ratio ± MD] [MA; mean log2-ratio ± SD]
rank ACC. no. Name gill 7 - day 3 gill 9 -day 3 gill 7 - day 7 gill 9 -day 7 gill 7 - 10 weeks gill 8 - day 7
1 DV944570.1 senescence-associated protein -0.12 ± 0.50 -0.03 ± 0.03 -1.16* ± 0.64 -0.07 ± 0.25 0.69 ± 0.57 -0.06 ± 0.03
2 DW250369.1 pg1 protein 1.01 ± 0.61 0.60 ± 1.14 -0.34 ± 0.49 -0.29 ± 0.56 - 1.644 ± 0.414
3 DN161290.1 unknown (putative Syntaxin binding protein 2) 0.32 ± 0.32 0.42 ± 0.10 0.88* ± 0.10 0.67 ± 0.31 0.49 ± 0.66 2.86 ± 0.02
4 DN796131.1 23S ribosomal RNA gene (a) -1.20 ± 0.92 -0.25 ± 0.18 -1.14 ± 1.16 -0.87* ± 0.46 - 0.36 ± 0.34
5 DW584691.1 calcium-activated chloride channel 0.25 ± 0.40 0.81* ± 0.34 -0.09 ± 0.30 0.16 ± 0.31 1.12 ± 0.88 -
6 DN635040.1 23S ribosomal RNA gene (b) -0.16 ± 1.42 0.09 ± 0.64 -0.80* ± 0.22 -0.27 ± 0.49 - 1.29 ± 0.16
7 DV944193.1 rRNA intron-encoded homing endonuclease 0.54 ± 1.00 0.80 ± 0.81 0.06 ± 0.22 0.59 ± 0.41 - 1.073 ± 0.38
8 DW249535.1 unknown -0.12 ± 1.43 0.04 ± 0.55 -0.71* ± 0.62 -0.41 ± 0.65 - 0.99 ± 0.29
9 DY656451.1 unknown -0.15 ± 0.23 0.05 ± 0.26 0.57* ± 0.09 0.04 ± 0.27 - -0.15 ± 0.30
10 DW251049.1 unknown -0.35 ± 0.24 -0.53* ± 0.28 -0.05 ± 0.22 -0.10 ± 0.19 - -0.86 ± 0.23
11 DN551127.1 unknown 0.54 ± 0.25 0.26 ± 0.14 0.48* ± 0.31 0.37 ± 0.48 - 2.34 ± 0.07
12 DW250828.1 thymosin-repeated protein 1 -0.39 ± 0.21 -0.46* ± 0.14 0.13 ± 0.21 0.19 ± 0.21 - -0.05 ± 0.06
13 DN202592.1 multispanning membrane protein (transmembrane 9 superfamily protein member 4) 0.35 ± 0.23 0.21 ± 0.14 0.46* ± 0.11 0.36 ± 0.34 - 2.15 ± 0.08
14 DW250260.1 hyperpolarization activated cyclic nucleotide-gated potassium channel-2 -0.26 ± 0.30 -0.14 ± 0.08 -0.46* ± 0.24 -0.14* ± 0.08 -0.13 ± 0.26 -0.11 ± 0.09
18 DV944030.1 tetraspanin 3 (Transmembrane super family 4 member 8) -0.22 ± 0.11 -0.41* ± 0.05 0.01 ± 0.27 0.09 ± 0.03 - -0.13 ± 0.06
25 DY307809.1 potential integrin alpha-7 -0.12 ± 0.36 0.26 ± 0.21 -0.35* ± 0.28 -0.03 ± 0.26 - -
29 DV642643.1 kazal-type proteinase inhibitor 0.25* ± 0.17 0.21* ± 0.12 0.33* ± 0.20 0.24* ± 0.15 0.12 ± 0.84 1.04 ± 0.11
31 DN635395.1 unknown -0.17* ± 0.04 -0.14* ± 0.04 -0.32* ± 0.06 -0.13* ± 0.06 0.25 ± 0.68 -0.11 ± 0.11
63 DV943872.1 predicted ATPase -0.34 ± 0.33 -0.24 ± 0.10 -0.58 ± 0.39 -0.23* ± 0.09 - -0.65 ± 0.23
88 CX994129.1 anion/bicarbonate transporter family member (SLC family 4, member 1) -0.31 ± 0.11 -0.20* ± 0.09 -0.22 ± 0.07 -0.22 ± 0.11 0.18 ± 0.54 0.41 ± 0.11
- DN739347.1 glycosyl-phosphatidylinositol-linked carbonic anhydrase VII -0.09 ± 0.05 -0.06 ± 0.06 -0.13* ± 0.07 -0.08* ± 0.03 0.40 ± 0.35 -0.92 ± 0.07
- DN551450.1 glutathione peroxidase 0.02 ± 0.06 0 ± 0.05 0.03* ± 0.02 -0.03 ± 0.05 -0.90 ± 0.24 -0.81 ± 0.14
- DY656042.1 vacuolar-type H+-ATPase subunit A -0.05 ± 0.37 0.11 ± 0.09 -0.11 ± 0.09 0.07 ± 0.06 0.45 ± 0.65 -0.17 ± 0.27
- DY657461.1 v-type proton ATPase subunit E 0.02 ± 0.03 -0.02 ± 0.04 0 ± 0.03 -0.02 ± 0.03 0.30 ± 0.39 -0.12 ± 0.17
- DW251095.1 cytoplasmic carbonic anhydrase 0.07 ± 0.01 -0.03 ± 0.06 0 ± 0.07 0.01 ± 0.01 -0.12 ± 1.20 1.78 ± 0.16
- DN202373.1 anion/bicarbonate transporter family member (SLC family 4, member 11) -0.02 ± 0.03 0.02 ± 0.06 -0.02 ± 0.10 0.01 ± 0.02 0.02 ± 0.64 -1.29 ± 0.47
- DN796352.1 K+/Cl- symporter (solute carrier family 12, member 6) -0.04 ± 0.04 0.22 ± 0.17 0.01 ± 0.04 -0.07 ± 0.07 0.91 ± 0.30 0.25 ± 0.23
- DV111894.1 Na+/K+/2Cl- cotransporter 0.05 ± 0.05 0.01 ± 0.05 0 ± 0.06 0 ± 0.10 0.62 ± 0.57 -0.20 ± 0.09
- DW250552.1 Na+/K+ ATPase alpha subunit 0.04 ± 0.05 0.02 ± 0.05 0.05 ± 0.10 0.03 ± 0.16 0 ± 1.11 1.38 ± 0.11
- DY308103.1 Na+/H+ exchanger (NHE 3) - - - - 0.27 ± 0.03 -0.13 ± 0.14
  1. Included are the 14 most strongly regulated transcripts (as identified by sign test and F-test), as well as several transcripts which have a potential function during structural modifications or in ion- and acid/base regulation. Transcripts are ordered according to the rank in absolute regulation. Accession numbers (ACC. no.) refer to the ESTs generated for the C. maenas microarray by Towle et al. [37] in the database GenBank (NCBI). Rank 1 = highest regulation; bold and underlined = significant regulation relative to the control identified by sign test; bold = identified to be differentially expressed by both tests; italic = identified to be differentially expressed only by F-test; "-" = a rank was not assigned in case the regulation was less than log2-ratio = |0.20|.