Table 10 List of primers used for real time RT-PCR.
From: Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus
SAPD ID | Gene Name | Accession Number | Primer sequence (5'→ 3') | Amplicon (bp) | Temp (°C) | Efficiency |
|---|---|---|---|---|---|---|
SAPD20351 | Beta-2-glycoprotein I precursor | IPI00298828 | F: TGGTTCGCCTCCTGTCTCC R: GGTTCTGGTGACTCATCCTCTG | 178 | 60 | 73.1% |
SAPD01318 | Collagen alpha1 I chain precursor | P02452 | F: AGACCTGCGTATCCCCAACTC R: GCCACCGTTCATAGCCTCTCC | 110 | 57 | 83.4% |
SAPD03530 | Collagen alpha2 V chain precursor | IPI00293881 | F: ACCTGTGACGACCTGAAGAGATGC R: TGGATGGGTTGGCGGAGATGC | 145 | 60 | 86.8% |
SAPD23304 | Cytochrome c | P81459 | F: AGGCATTCGTCCAGAAGTGTG R: TGGCATCGGTGTAGGAGTAGC | 132 | 56 | 84% |
SAPD05261 | IFI56 | Q7T2R0 | F: ACCTCGCTGCTCAGTACCTC R: GCCTCCTCCGCCAAATCAATG | 184 | 57 | 87.7% |
SAPD02730 | Glutathione S-transferase A5 | Q7RTV2 | F: AGACGTGCTGCTGCTTGAATGC R: TGGCTTCGGCTTCCTCTTGCTG | 157 | 60 | 99.1% |
SAPD00344 | P22phox protein | NP_001027717 | F: ATGCTTGCCACCGTCCTG R: TCTTGATGCTCTCTGCGACTG | 139 | 60 | 82.4% |
SAPD02155 | Proliferating cell nuclear antigen (pCNA) | P12004 | F: GAGCAGCTGGGTATTCCAGA R: CTGTGGCGGAGAACTTGACT | 148 | 60 | 83.4% |
SAPD13946 | Retinol-binding protein I, cellular | P82980 | F: TCCGCACCATAACCACCTTCAAG R: CCAGCCTCGTCCTTCCTTCTCC | 168 | 60 | 78.6% |
SAPD26722 | SPP1 protein | NP_001002308 | F: AGGTTGCTGACAGTTCTGAGAG R: GCGGCTGCTGCTACAATG | 130 | 57 | 92.2% |
--- | Ribosomal protein S18 | AM490061 | F: AGGGTGTTGGCAGACGTTAC R: CTTCTGCCTGTTGAGGAACC | 164 | 60 | 92.1% |