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Table 9 Comparison of Fold-change values from qPCR target genes and microarray in the 3 day sampling.

From: Skin healing and scale regeneration in fed and unfed sea bream, Sparus auratus

   WS ST STWS ST vs STWS
SAPD ID Target Transcript qPCR Probe 1 Probe 2 qPCR Probe 1 Probe 2 qPCR Probe 1 Probe 2 qPCR Probe 1 Probe 2
SAPD03530 Collagen V alpha2 1.087* 0.780 0.758 0.836 0.814 0.782 0.646 0.598 0.580 0.772 0.734 0.741
SAPD01318 Collagen I alpha1 0.838 0.398 0.547 1.294* 0.570 0.770 0.467 0.281 0.413 0.361 0.492 0.536
SAPD26722 SPP1 0.552 0.116 0.130 1.109* 0.415 0.477 0.501 0.293 0.323 0.451 0.708 0.678
SAPD00344 p22phox protein 0.574 0.255 0.250 0.367 0.296 0.303 0.380 0.231 0.231 1.035* 0.780 0.763
SAPD13946 Retinol-binding protein I 3.097 1.738 2.117 0.877 0.680 0.765 1.840 2.023 2.329 2.098 2.976 3.044
SAPD02730 Glutathione S-transferase A5 1.846 1.774 1.726 0.697 0.873 0.900 2.168 2.080 1.896 3.112 2.384 2.017
SAPD23304 Cytochrome c 1.373 1.874 1.949 0.625 0.873 0.845 2.149 2.180 2.278 3.438 2.498 2.696
SAPD20351 Beta-2-glycoprotein I 4.507 2.292 2.698 6.490 9.503 10.862 3.492 3.870 4.304 0.538 0.407 0.396
SAPD05261 IFI56 2.144 3.291 3.213 0.372 0.422 0.417 1.385 2.193 1.906 3.728 5.202 4.574
SAPD02155 pCNA 1.329 1.754 1.62 0.558 0.517 0.489 1.598 1.692 1.585 2.862 3.273 3.240
  1. Comparisons were made for each treatment in relation to the control with the additional comparison of fasted vs. fasted without scales (ST vs. STWS). Values correspond to the Fold-change which was calculated by the ratio of mean expression values between treatment and control groups (individual biological replicates of n = 5 for both qPCR and microarray). * indicate where differences between qPCR and microarray data were observed.