Mitochondrial genome sequencing helps show the evolutionary mechanism of mitochondrial genome formation in Brassica

Table 3 Function-unknown ORFs predicted by ORF Finder in six Brassica mitotypes

ORF	cam	pol	nap	ole	car	class	ORF	cam	pol	nap	ole	car	class
orf101c	+	+	+	2+	-	GR	orf106a	-	-	+	2+	+	M
orf265	+	+	orf261	orf266*(2+)	orf277	GR	orf108a	+	+	+	+	-	M
orf305	+	+	orf322*	orf311(2+)	orf310*	GR	orf110	+	+	+	2+	-	M
orf101d	+	+	+	2+	-	ID	orf112	+	+	+	2+	-	M
orf117b	-	-	+	2+	+	ID	orf113b	+	+	+	2+	orf170	M
orf122	+	+	-	2+	-	ID	orf114	+	+	+	-	+	M
orf124	-	-	-	-	+	ID	orf115b	+	+	+	2+	-	M
orf128	+	+	+	2+	-	ID	orf117a	+	+	+	+	-	M
orf132	+	+	-	2+	-	ID	orf118	+	+	orf124	orf120a	orf123a	M
orf138a	-	-	-	-	+	ID	orf123	+	+	+	2+	-	M
orf188	-	-	+	-	-	ID	orf125	+	+	+	2+	orf108c	M
orf195	+	+	+	-	-	ID	orf128a	-	-	-	-	+	M
orf222 ^#	-	orf224	orf222	-	-	ID	orf131	-	-	-	-	+	M
orf100a	+	+	+	2+	orf100c	M	orf147	+	+	+	+	orf141	M
orf101e	+	+	+	2+	-	M	orf164	+	+	+	+	-	M
orf101f	+	+	+	+	-	M	orf165	-	-	-	-	+	M
orf105	-	-	-	2+	-	M	orf293	+	+	orf286	-	orf288	M
Total number of function-unknown orf in six mitotypes								24 (44)	25 (45)	26 (46)	24 (44)	16 (36)

Each line presents the homologous ORFs. +, present; -, absent; GR, gene rearrangement; ID, insertion/deletion; M, mutation. ORF copy numbers are shown ahead of +. Class shows the cause of change in each line.
* indicates an exception for class and that the cause is mutation.
^# orf222 and orf224 are the same as Table 1.
Data for pol and nap are from our previous report and Handa's results [6], respectively. The total numbers outside parentheses show the changing ORF for each mitotype; the number in parentheses contains the ORFs that exist consistently in the six mitotypes. The total number of ORFs in ole is not plus the multiple copies, if containing the copies the total number is 73.

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