Figure 1

Time dependent gene expression changes induced by hypoxia. (A) Time course of expression of selected HIF-1 alpha target genes in all brain regions studied derived from the microarray experiments. Adm: Adrenomedullin. Vegfa: Vascular endothelial growth factor A. The y-axis is the expression value (normalized to the sham controls and log-transformed). The x-axis shows the time course of gene expression in the following order: C, control; H1, 1 hour of hypoxia; H3, 3 hours of hypoxia; R1, 1 hour after reoxygenation; R3, 3 hours after reoxygenation; R6, 6 hours after reoxygenation; R12, 12 hours after reoxygenation; R24, 24 hours after reoxygenation. Regions: cerebral cortex (Cortex), hippocampus (Hippo.), striatum (Stri.), thalamus (Thal.), midbrain (Mid.), pons and medulla (Pon.), cerebellum (Cere.). (B). Total number of up-regulated transcripts and total number of down-regulated transcripts in the entire brain. The y-axis is the number of up-regulated transcripts (red line) or down-regulated transcripts (blue line) summed across all brain regions at each time point. The x-axis shows the time courses following hypoxia. (C). Venn diagram of time-dependent gene expression changes in cerebral cortex from H1 through R1. These genes were derived by performing a one-way ANOVA with a False Discovery Rate of 5% and with a fold change of ≥ 1.5 or greater.