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Table 2 Hits organized by genes in the K/P JAK/STAT screen.

From: False negative rates in Drosophila cell-based RNAi screens: a case study

Gene

Number of dsRNAs

Number of scoring dsRNAs

Number of non-scoring dsRNAs

Category

Expressed in S2R+ Cells

Abl

4

4

0

1

Yes

CycA

3

3

0

1

Yes

dome

3

3

0

1

Yes

hop

3

3

0

1

Yes

mts

2

2

0

1

Yes

CycE

3

2

1

2

Yes

Pp4-19C

4

2

2

2

Yes

CG17090

3

2

1

2

Yes

puc

3

2

1

2

Yes

CG34318 | CG8179

4

1

3

3

No

CanA1

3

1

2

3

No

CG4839

3

1

2

3

No

CG7597

3

1

2

3

Yes

CG9389

3

1

2

3

No

mtm

3

1

2

3

Yes

Pi3K21B

3

1

2

3

Yes

smi35A

3

1

2

3

Yes

Src42A

3

1

2

3

Yes

CG8509

2

1

1

3

No

gskt

2

1

1

3

No

htl

2

1

1

3

No

Myt1

2

1

1

3

Yes

Pp1-Y2

2

1

1

3

No

S6k

2

1

1

3

Yes

  1. Genes were included on this list if at least one dsRNA yielded a Z-score of +/- 2 or better across both replicates. The number of dsRNAs in the K/P set is indicated as well as the number of those dsRNAs that were scored in the screen with a Z-score with an absolute value of 1.5 or better. Genes were binned into 3 categories: Category 1 contains genes where 4 out of 4 or 3 out of 3 or 2 out of 2 dsRNAs scored. Category 2 contains genes where 2 out of 3 or 2 out of 4 or 3 out of 4 dsRNAs scored. Category 3 contains genes where only 1 dsRNA scored. A gene was defined to be expressed in S2R+ if the FPKM value was greater than 1.