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Table 3 Model of RNAi reagent disambiguation methods under one, two or three reagents per gene.

From: False negative rates in Drosophila cell-based RNAi screens: a case study

  1 RNAi Reagent/Gene 2 RNAi Reagents/Gene 3 RNAi Reagents/Gene
"Lenient" Rule A: Number of False Negatives R FN × H R F N 2 × H R F N 3 × H
"Lenient" Rule A: Number of False Positives R FP × N ( R F P × 2 - R F P 2 ) × N ( R F P × 3 - R F P 2 × 3 + R F P 3 ) × N
"Stringent" Rule B: Number of False Negatives R FN × H ( R F N × 2 - R F N 2 ) × H ( R F N × 3 - R F N 2 × 3 + R F N 3 ) × H
"Stringent" Rule B: Number of False Positives R FP × N R F P 2 × N R F P 3 × N
"Balanced" Rule C: Number of False Negatives R FN × H ( R F N × 2 - R F N 2 ) × H ( R F N 2 × 3 - R F N 3 × 2 ) × H
"Balanced" Rule C: Number of False Positives R FP × N R F P 2 × N ( R F P 2 × 3 - R F P 3 × 2 ) × N
  1. N = The number of genes in the screening library, H = The number of genes a screen should uncover under ideal conditions, RFN = Fraction of reagents of H that fail. RFP = Fraction of reagents of N that are false positives. Rule "A": Only one reagent targeting a gene need to be a "hit" for the gene to be called a "hit". Rule "B": All reagents targeting a gene need to be a "hit". Rule "C": More than half of reagents must score as a "hit".