From: False negative rates in *Drosophila* cell-based RNAi screens: a case study

1 RNAi Reagent/Gene | 2 RNAi Reagents/Gene | 3 RNAi Reagents/Gene | |
---|---|---|---|

"Lenient" Rule A: Number of False Negatives |
R_{
FN
} × H
| ${R}_{FN}^{2}\times H$ | ${R}_{FN}^{3}\times H$ |

"Lenient" Rule A: Number of False Positives |
R_{
FP
} × N
| $\left({R}_{FP}^{}\times 2-{R}_{FP}^{2}\right)\times N$ | $\left({R}_{FP}^{}\times 3-{R}_{FP}^{2}\times 3+{R}_{FP}^{3}\right)\times N$ |

"Stringent" Rule B: Number of False Negatives |
R_{
FN
} × H
| $\left({R}_{FN}\times 2-{R}_{FN}^{2}\right)\times H$ | $\left({R}_{FN}\times 3-{R}_{FN}^{2}\times 3+{R}_{FN}^{3}\right)\times H$ |

"Stringent" Rule B: Number of False Positives |
R_{
FP
} × N
| ${R}_{FP}^{2}\times N$ | ${R}_{FP}^{3}\times N$ |

"Balanced" Rule C: Number of False Negatives |
R_{
FN
} × H
| $\left({R}_{FN}\times 2-{R}_{FN}^{2}\right)\times H$ | $\left({R}_{FN}^{2}\times 3-{R}_{FN}^{3}\times 2\right)\times H$ |

"Balanced" Rule C: Number of False Positives |
R_{
FP
} × N
| ${R}_{FP}^{2}\times N$ | $\left({R}_{FP}^{2}\times 3-{R}_{FP}^{3}\times 2\right)\times N$ |