Temporal transcriptome changes induced by MDV in marek's disease-resistant and -susceptible inbred chickens

BMC Genomics

Table 4 Validation of microarray results by quantitative PCR

Genes	Probe Name in Micro-array	Time points	Gene expression fold change after MDV infection in each lines (Inf./Non.)
			line 6₃		line 7₂		RCS-M
			Micro- array	Q-PCR	Micro- array	Q-PCR	Micro-Array	Q-PCR
		5dpi	1.02	*1.45*	2.85	*4.07*	1.41	1.29
CTLA-4	*019882	10dpi	2.31	*3.37*	*3.29*	*13.68*	*3.56*	*7.02*
		21dpi	0.62	*2.05*	*3.07*	2.03	1.27	1.44
		5dpi	1.46	1.05	1.41	1.19	1.52	0.85
CD8α	*007686	10dpi	*2.92*	*2.33*	1.51	1.35	*3.90*	2.80
		21dpi	1.08	*3.13*	*0.35*	*0.05*	1.24	2.01
		5dpi	1.17	1.14	1.07	0.97	1.14	0.78
CD8β	A_87_P008699	10dpi	*1.87*	*2.86*	1.03	2.05	*1.86*	2.30
		21dpi	1.42	*3.31*	*0.32*	*0.04*	1.10	*2.03*
		5dpi	1.24	*3.37*	4.09	4.13	1.43	1.68
USP18	*005670	10dpi	2.07	2.15	*4.87*	*5.55*	2.53	0.58
		21dpi	0.98	11.89	*3.28*	4.54	*2.73*	*5.99*
		5dpi	1.00	2.85	5.67	*9.36*	1.12	*4.17*
TNFRSF6B	*003404	10dpi	*5.03*	*9.47*	*5.52*	*11.46*	2.75	2.35
		21dpi	1.54	*5.27*	*4.28*	*9.14*	1.00	*6.85*
		5dpi	1.00	0.64	0.60	*0.28*	*0.62*	*0.29*
MMP2	A_87_P009159	10dpi	0.78	0.89	*0.63*	0.93	*0.54*	0.62
		21dpi	0.88	1.10	*0.22*	*0.02*	0.82	1.16
		5dpi	1.00	0.72	1.04	*0.18*	1.71	0.91
IL8	*010230	10dpi	*8.28*	0.66	*3.01*	*0.19*	*4.25*	*0.10*
		21dpi	18.51	2.22	*0.46*	*0.02*	0.81	*2.59*
		5dpi	1.00	*3.14*	1.00	*0.45*	1.85	0.81
GHR	A_87_P009190	10dpi	*8.57*	*1.25*	0.24	*1.82*	1.00	*0.53*
		21dpi	1.54	*1.56*	0.40	0.59	1.00	*2.76*

The numbers here represent the fold change of gene expression after MDV infection. The numbers equal to 1.00 means the expression level doesn't change after MDV infection. The numbers > 1.00 means the expression level is increased after MDV infection while numbers < 1.00 indicate that the expression level is reduced after MDV infection. The numbers in bold and italic are statistically significant (P < 0.05).

ISSN: 1471-2164