Deep sequencing-based analysis of the anaerobic stimulon in Neisseria gonorrhoeae

Table 3 Genes of the cryptic plasmid found to be differentially expressed in response to anaerobiosis.

Gene	Replicate 1^b	Replicate 2	Definition^a
Transcript 1
ORF1	12.8	9.7	Putative plasmid replicase, RepA
ORF2	4.5	3.1	Hypothetical protein
ORF3	4.5	3.4	Hypothetical protein
ORF4	4.5	2.6	Putative plasmid antitoxin, VapX
ORF5	5.1	5.5	Putative plasmid toxin, VapD
Transcript 2
ORF6	18.2	6.0	Hypothetical protein
ORF7	13.3	34.4	Hypothetical protein
cppC	19.9	41.6	Putative plasmid mobilization protein, MobA
cppB	6.2	7.9	Putative plasmid mobilization protein, MobB, partial only
cppA	7.6	8.2	Cryptic plasmid protein A

^a Protein definitions are derived from Korch et al., (1985) [80] or the NCBI conserved domain database [33].
^b The fold change for each replicate was calculated by comparing total number of reads for each gene in anaerobically grown gonococci to number of reads for each gene in aerobically grown gonococci. Values were normalized to total number of chromosomal reads. A positive value represents an induction of gene expression anaerobically, and a negative value represents a repression of gene expression anaerobically.