RNAi sensitivity is a complex trait mostly associated to ppw-1 polymorphisms. A) We genotyped 31 RILs at the ppw-1 locus and addressed whether each of these RILs are RNAi sensitive or not using FCs from mel-26(RNAi), pos-1(RNAi), par-1(RNAi), smo-1(RNAi), par-6(RNAi), and gld-1(RNAi). This analysis has revealed that 25.8% of RILs cannot be categorized as such, underlying that the RNAi sensitive trait is complex. However, 74.2% of these could be categorized as either N2-like or CB-like. B) From the RILs that can be categorized, most cases (82,6%), can be associated to the correct ppw-1 allele. On the other hand, only 17.4% of the cases are not significantly associated with ppw-1. Therefore, ppw-1 is a strong predictor of RNAi sensitivity, but not the only one.