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Table 1 Numerical summary for Illumina Assay-2

From: Next-generation DNA sequencing-based assay for measuring allelic expression imbalance (AEI) of candidate neuropsychiatric disorder genes in human brain

Stage

gDNA/RNA

Number

0

Candidate genes

74

0

Independent brain samples

52

0

Genotypes

52 × ~1.14 × 106

1

Isolated gDNA/cDNA

2 × 52 tubes

2

Individual PCR products

2762 tubes

2'

Supplemental PCR products

~200 tubes

3

Pooled, sample-specific PCR products

2 × 52 tubes

4

Sample-specific PCR products ligated to indexed Illumina adapters

2 × 52 tubes

5

Complete set of PCR products ligated to indexed Illumina adapters

2 × 1 tube

6

AEI Ratios

1,371 gDNA

1,313 cDNA