Stage | gDNA/RNA | Number |
---|---|---|
0 | Candidate genes | 74 |
0 | Independent brain samples | 52 |
0 | Genotypes | 52 × ~1.14 × 106 |
1 | Isolated gDNA/cDNA | 2 × 52 tubes |
2 | Individual PCR products | 2762 tubes |
2' | Supplemental PCR products | ~200 tubes |
3 | Pooled, sample-specific PCR products | 2 × 52 tubes |
4 | Sample-specific PCR products ligated to indexed Illumina adapters | 2 × 52 tubes |
5 | Complete set of PCR products ligated to indexed Illumina adapters | 2 × 1 tube |
6 | AEI Ratios | 1,371 gDNA 1,313 cDNA |