454 read abundance and qPCR based estimates of relative expression in response to 2 salinity treatments. (Main panel) comparison of relative gene expression (normalised to actin and alpha-tubulin) determined from qPCR assays and 454 read abundance. Target gene abbreviations are as follows: Cys - cysteine proteinase, phNMT - phosphoethanolamine N-methyltransferase, 40Sr - 40S ribosomal protein, Btub - beta tubulin, CCP - cathepsin B cysteine proteinase, Crt - Calreticulin, HSP90 - heat shock protein 90 - numbers refer to contig ID where identifications were not made. * indicates significant over-expression based on qPCR assays (p < 0.05). (Inset) Mean specific growth rate responses of triplicate O. marina cultures grown at 30 and 50 PSU; error bars are standard deviations.