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Table 3 Comparison of differential gene expression as determined by microarray hybridization and qRT-PCR

From: Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses

Gene IDa Typeb Microarray qRT-PCR
   log2FCc q d log2FCc p e
CYP51      
FGSG_01000 CYP51B 2.96 0.0303 2.89 < 0.0001
FGSG_04092 CYP51A 7.35 0.0061 6.92 < 0.0001
FGSG_11024 CYP51C 2.06 0.0385 1.54 0.0001
ABC transporters      
FGSG_00046 MRP 3.00 0.0392 1.17 0.0231
FGSG_02786 MDR 2.16 0.0392 1.64 0.0036
FGSG_03032 MDR (half) -1.29 0.0392 -0.99 0.0793
FGSG_03882 PDR -3.65 0.0516 -3.27 0.0560
FGSG_05076 PDR (half) 1.94 0.0385 1.17 0.0249
FGSG_06771 MDR 2.02 0.0392 1.56 0.0017
FGSG_06881 MDR 1.38 0.0401 2.08 0.0303
FGSG_07325 MRP 1.57 0.0539 1.42 0.0033
FGSG_08308 MRP 4.07 0.0508 3.41 0.0310
FGSG_08309 PDR -2.95 0.0509 -2.05 0.0686
FGSG_08312 PDR 2.67 0.0385 1.90 < 0.0001
FGSG_08373 n.d. 1.55 0.0395 1.32 0.0036
FGSG_08830 PDR 6.58 0.0523 3.78 0.0226
FGSG_10995 MRP 2.61 0.0497 1.30 0.0176
FGSG_11028 MRP 1.44 0.0502 -0.85 0.4518
FGSG_11988 MDR -1.91 0.0509 -1.63 0.0264
Transcription factors      
FGSG_00069 Zn2Cys6 -2.14 0.0385 -1.81 0.0207
FGSG_01293 Zn2Cys6 2.08 0.0385 1.91 0.0010
FGSG_01341 C2H2 1.62 0.0357 1.92 0.0042
FGSG_01669 Zn2Cys6 3.86 0.0392 3.30 0.0002
FGSG_05949 WING 4.16 0.0385 3.96 < 0.0001
FGSG_06324 Zn2Cys6 1.64 0.0385 1.00 < 0.0001
FGSG_06810 Zn2Cys6 1.70 0.0387 1.35 0.0002
FGSG_09333 Zn2Cys6 -1.22 0.0385 -0.57 0.0121
FGSG_09349 Zn2Cys6 -2.16 0.0385 -0.98 0.0092
FGSG_10470 C2H2 4.74 0.0385 4.32 < 0.0001
FGSG_10914 Zn2Cys6 -1.03 0.0392 -1.14 0.0738
FGSG_11561 Zn2Cys6 1.48 0.0387 1.57 0.0162
  1. a The given gene ID is the entry number of the F. graminearum genome annotation FG3. b Classification to gene subfamilies. c The relative transcriptional differences between fungicide-treatment vs. untreated control given as log2 transformed fold-change as determined by microarray hybridization and by qRT-PCR. d Statistical assessment of transcriptional differences by ODP. The q value is a measure of the false-positive discovery rate in a given set of genes. e Statistical assessment of transcriptional differences by Student's t-test.
  2. ALDp, adrenoleukodystrophy protein; CYP51, cytochrome P450 sterol 14α-demethylase; MDR, multi drug resistance proteins; MRP, multidrug resistance-associated proteins; PDR, pleiotropic drug resistance proteins; C2H2, Cys-Cys-His-His motif zinc finger proteins; WING, winged helix-turn-helix DNA-binding protein; Zn2Cys6, Zn(II)2-Cys6 binuclear cluster domain-type transcription factors; n.d. not defined.