Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses

Table 3 Comparison of differential gene expression as determined by microarray hybridization and qRT-PCR

Gene ID^a	Type^b	Microarray		qRT-PCR
		log₂FC^c	q ^d	log₂FC^c	p ^e
CYP51
FGSG_01000	CYP51B	2.96	0.0303	2.89	< 0.0001
FGSG_04092	CYP51A	7.35	0.0061	6.92	< 0.0001
FGSG_11024	CYP51C	2.06	0.0385	1.54	0.0001
ABC transporters
FGSG_00046	MRP	3.00	0.0392	1.17	0.0231
FGSG_02786	MDR	2.16	0.0392	1.64	0.0036
FGSG_03032	MDR (half)	-1.29	0.0392	-0.99	0.0793
FGSG_03882	PDR	-3.65	0.0516	-3.27	0.0560
FGSG_05076	PDR (half)	1.94	0.0385	1.17	0.0249
FGSG_06771	MDR	2.02	0.0392	1.56	0.0017
FGSG_06881	MDR	1.38	0.0401	2.08	0.0303
FGSG_07325	MRP	1.57	0.0539	1.42	0.0033
FGSG_08308	MRP	4.07	0.0508	3.41	0.0310
FGSG_08309	PDR	-2.95	0.0509	-2.05	0.0686
FGSG_08312	PDR	2.67	0.0385	1.90	< 0.0001
FGSG_08373	n.d.	1.55	0.0395	1.32	0.0036
FGSG_08830	PDR	6.58	0.0523	3.78	0.0226
FGSG_10995	MRP	2.61	0.0497	1.30	0.0176
FGSG_11028	MRP	1.44	0.0502	-0.85	0.4518
FGSG_11988	MDR	-1.91	0.0509	-1.63	0.0264
Transcription factors
FGSG_00069	Zn2Cys6	-2.14	0.0385	-1.81	0.0207
FGSG_01293	Zn2Cys6	2.08	0.0385	1.91	0.0010
FGSG_01341	C2H2	1.62	0.0357	1.92	0.0042
FGSG_01669	Zn2Cys6	3.86	0.0392	3.30	0.0002
FGSG_05949	WING	4.16	0.0385	3.96	< 0.0001
FGSG_06324	Zn2Cys6	1.64	0.0385	1.00	< 0.0001
FGSG_06810	Zn2Cys6	1.70	0.0387	1.35	0.0002
FGSG_09333	Zn2Cys6	-1.22	0.0385	-0.57	0.0121
FGSG_09349	Zn2Cys6	-2.16	0.0385	-0.98	0.0092
FGSG_10470	C2H2	4.74	0.0385	4.32	< 0.0001
FGSG_10914	Zn2Cys6	-1.03	0.0392	-1.14	0.0738
FGSG_11561	Zn2Cys6	1.48	0.0387	1.57	0.0162

^a The given gene ID is the entry number of the F. graminearum genome annotation FG3. ^b Classification to gene subfamilies. ^c The relative transcriptional differences between fungicide-treatment vs. untreated control given as log₂ transformed fold-change as determined by microarray hybridization and by qRT-PCR. ^d Statistical assessment of transcriptional differences by ODP. The q value is a measure of the false-positive discovery rate in a given set of genes. ^e Statistical assessment of transcriptional differences by Student's t-test.
ALDp, adrenoleukodystrophy protein; CYP51, cytochrome P450 sterol 14α-demethylase; MDR, multi drug resistance proteins; MRP, multidrug resistance-associated proteins; PDR, pleiotropic drug resistance proteins; C2H2, Cys-Cys-His-His motif zinc finger proteins; WING, winged helix-turn-helix DNA-binding protein; Zn2Cys6, Zn(II)2-Cys6 binuclear cluster domain-type transcription factors; n.d. not defined.

ISSN: 1471-2164