Transcriptomic effects of di-(2-ethylhexyl)-phthalate in Syrian hamster embryo cells: an important role of early cytoskeleton disturbances in carcinogenesis?

Landkocz, Yann; Poupin, Pascal; Atienzar, Franck; Vasseur, Paule

doi:10.1186/1471-2164-12-524

Table 1 List of genes identified by Differential Display after DEHP exposure and classified by major biological function, according to GO process

From: Transcriptomic effects of di-(2-ethylhexyl)-phthalate in Syrian hamster embryo cells: an important role of early cytoskeleton disturbances in carcinogenesis?

Id	Official symbol	Gene Name	Accession Nr	Tblastx expect	Effect of DEHP
1-Transcription and Signal Transduction
A0601	Hsp90	Heat shock protein 90 kDa protein	NM_010478	9E-25	+
A0602	Hsp70	Heat shock protein 70 kDa protein	NM_005346	1E-24	+
A3201	Cebpd	CCAAT/enhancer binding protein delta	NM_005195	9E-10	-
A4102	Ncbp2	nuclear cap binding protein subunit 2	NM_007362	6E-21	+
A5101	Mettl5	methyltransferase like 5	NM_029280	7E-10	+
A5102	Ogt	O-linked N-acetylglucosamine transferase	NM_181672	5E-28	+
A6101 A6102	Nr1i2	nuclear receptor subfamily 1I2	NM_003889	1E-158 3E-74	+
A6302	Mapk15	mitogen-activated protein kinase 15	NM_177922	3E-16	+
A6801	Rab1b	member of RAS oncogene family	NM_030981	1E-22	++
A7502	Mdb1	methyl-CpG binding domain protein 1	NM_013594	2E-16	--
C0602	Gper	G protein-coupled estrogen receptor 1	NM_001098201	6E-68	+
C1001	Hmbox1	Homeobox 1	NM_024567	2E-32	++
C1002	Mapk3	mitogen-activated protein kinase 3	NM_001109891	9E-60	-
C1301	Usp3	Ubiquitin peptidase 3	NM_006537	9E-34	+
C1401	Psmc5	26S protease regulatory subunit ATPase 5	NM_002805	2E-23	-
C4601	Hsph1	heat shock 105kDa/110kDa protein	NM_006644	5E-07	+
C4802	Irf2	interferon regulatory factor 2	NM_008391	8E-19	+
C7101	Lmo4	LIM domain only 4	NM_006769	7E-09	+
G0301	Smarcc1	SWI/SNF related actin dependent regulator of chromatin	NM_003074	3E-20	-
G1001	Chd4	ATP dependant hélicase 4	NM_001273	1E-45	-
G1504	Foxp1	Forkhead box P1	NM_032682	3E-50	++
G1701	Creb3l1	cAMP responsive element binding protein 3-like 1	NM_052854	8E-19	+
G1902	Pbrm	Polybromo domain	NM_001081251	8E-49	+
G3301	Rxfp2	relaxin/insulin-like family peptide receptor 2	NM_130806	1E-24	+
G3501	Akap5	A kinase anchor protein 5	NM_004857	1E-05	+
G3801 G7001	Eif1	eukaryotic translation initiation factor 1	NM_005801	3E-42 1E-15	+
G4801	Spry3	sprouty homolog 3	NM_005840	0.0002	+
G6001	Foxa3	forkhead box A3	NM_008260	9E-17	-
G6201	Mett5d1	methyltransferase 5 containing 1	NM_029790	0.0038	+
2-Regulation of cytoskeleton
A0201	Cttnbp2	Cortactin binding protein 2	NM_030249	0.0001	+
A1901	Snx6	sorting nexin 6	NM_021249	8E-10	-
A2401	Lrrc8a	leucine rich repeat containing 8A	NM_177725	5E-32	-
A3501 A3502 C3501 G3502 G3503	Actb	beta-actin	NM_007393	3E-51 1E-106 2E-21 7E-114 3E-48	+
A3702	Col1a1	collagen, type I, alpha 1	NM_000088	1E-21	+
A3801	Nrp2	neuropilin 2	NM_0010774	1E-22	-
A5201	Ctnnbip1	catenin beta interacting protein 1	NM_020248	0.0008	-
A5202	Enah	enabled homolog	NM_008680	2E-09	-
A5402	Kif23	kinesin family member 23	NM_024245.4	1E-25	++
A7501	Cdh3	cadherin 3	NM_007665	0.0007	-
C4502	Nid2	nidogen 2	NM_008695	0.006	-
C6603 C6604 C6605	Crip1	cysteine-rich protein 1	NM_007763	1E-31 9E-08 3E-61	+
G0601	Thy1	Thy-1 cell surface antigen	NM_009382.3	0.001	-
G0801	Calml3	calmodulin-like 3	NM_027416.3	0.0004	+
G1102	Flrt2	fibronectin leucine rich transmembrane protein 2	NM_201518	2E-08	-
G1301	Has2	hyaluronan synthase 2	NM_008216	8E-18	-
G1901	Plekha5	Pleckstrin homology domain A5	NM_019012	3E-06	+
G2301	Thbs1	thrombospondin 1	NM_011580	3E-20	-
G4001	Coro1C	coronin, actin binding protein	NM_014325	4E-07	+
G4802	Tubb2b	Tubulin beta	NM_023716	0.0001	+
G6901	Dclk1	doublecortin-like kinase 1	NM_019978	1E-06	+
3-Xenobiotic metabolism
A0901	Cyp2e1	Cytochrome P450 2e1	NM_021282	3E-44	+
A2901	Ephx1	Epoxide Hydrolase 1	NM_000120	4E-21	+
A5701	Gstp1	glutathione S-transferase, pi 1	NM_000852	1E-24	+
A6601	Gstt1	glutathione S-transferase, theta 1	NM_008185	1E-44	-
C0101 C1501 G1502	Txnrd1	Thioredoxin reductase 1	NM_001042523	1E-20 1E-14 3E-79	+
C2901	Cyp1b1	Cytochrome P450 1b1	NM_009994	0.0	+
C3101	Gstm5	glutathione S-transferase, mu 5	NM_010360	4E-51	-
C3701	Tnfa	tumor necrosis factor-alpha	NM_011659	0.00009	+
G0101	Psme4	proteasome activator subunit 4	NM_134013	4E-87	+
G0701	Mt2a	metallothionein 2A	NM_005953	7E-20	-
G1201	Ggt1	gamma-glutamyltransferase 1	NM_013430	3E-46	+
G1501	Txnrd2	Thioredoxin reductase 2	NM_006440	9E-19	+
G2001	Pdia4	Disulfite Isomerase 4	NM_004911	5E-46	+
G2202	Ahcy	S-adenosylhomocystein hydrolase	NM_016661	1E-10	-
G2302	Cyp2f2	Cytochrome P450 2f2	NM_007817	9E-25	--
G3302	Cytb	Mesocricetus auratus cytochrome b	YP_003208313	9E-86	++
4-Apoptosis
A4301	Pik3r1	phosphatidylinositol 3-kinase	NM_001024955	1E-80	+
C1701	Tp53	tumor supressor p53	U07182	6E-83	-
C7103 G3701	Bcl10	B-cell CLL/lymphoma 10	NM_003921	4E-37 5E-82	+
G1503	Nfkb1	NF-κB	NM_008689	2E-102	+
G2201	Casp8	Caspase 8	NM_009812	6E-29	-
G2305	Eef1d	Eukaryotic translation elongation factor 1 delta	NM_029663	3E-17	+
G3001	Topors	topoisomerase I p53-binding	NM_134097	4E-10	-
G4101 G4102	Sh3kbp1	SH3-domain kinase binding protein 1	NM_031892	1E-09 0.0008	+
G5301	Cmyc	myelocytomatosis oncogene	AJ582076	2E-27	-
5-Lipidogenesis
A0501	Pla2g2d	phospholipase A2, group IID	NM_011109	0.00003	+
A3003	Dhcr7	dehydrocholesterol reductase	NM_007856	3E-36	+
C0701	Scl27a1	solute carrier family 27A1	NM_198580	0.0	+
C0901	Acaa1	Acetyl-CoA acyltransferase 1	NM_130864	1E-77	+
C4602	Lpl	lipoprotein lipase	NM_008509	1E-37	-
G0201	Star	steroidogenic acute regulatory protein	NM_011485	7E-08	-
6-Protein conformation or transport
A3801	Rpn1	ribophorin I	NM_133933	3E-14	-
A4701	Ergic3	ERGIC and golgi 3	NM_198398	6E-21	+
A5302	Gxylt1	glycosyltransferase 8 domain containing 3	NM_173601	0.0002	+
C0601	Ppia	peptidylprolyl isomerase A	NM_021130	7E-71	+
C2001	Grpel1	GrpE-like 1, mitochondrial	NM_024478	1E-13	+
C2201	Fxn	frataxin	NM_008044	4E-33	+
C3602	Slc26a9	solute carrier family 26, member 9	NM_177243	1E-9	+
C7501	Slc13a3	solute carrier family 13 member 3	NM_054055	3E-29	-
G1801	Slc15a1	Solute carrier family 15 member 1	NM_053079	0.0002	-
G3702	Rpn2	ribophorin II	NM_019642	3E-26	-
G6101	Nrbp1	nuclear receptor binding protein 1	NM_013392	4E-12	+
G6601	Slc6a8	solute carrier family 6 member 8	NM_005629	0.0001	-
7-Cell cycle
A0401	Ingap	Mesocricetus auratus islet neogenesis associated protein	U41738	4E-79	-
A3002	Cdkn2b	cyclin-dependent kinase inhibitor 2B	NM_004936	1E-9	-
A4801	Ppp1cc	protein phosphatase 1, catalytic subunit, gamma	NM_002710	2E-78	+
A5702	Sec11a	SEC11 homolog A	NM_014300	1E-19	+
C4002	Mapk4	mitogen-activated protein kinase 4	NM_002747	9E-26	+
C4701	Ccndbp1	cyclin D-type binding-protein 1	NM_010761	1E-17	+
C6301 C6302	Zw10	ZW10 homolog centromere/kinetochore protein	NM_004724	1E-04 1E-05	-
8-Other functions
A3903	Rexo2	REX2, RNA exonuclease 2 homolog	NM_015523	6E-21	-
A5403	Hk2	hexokinase 2	NM_000189	4E-28	+
A5602	Pcbp2	poly(rC)-binding protein 2	NM_031989	1E-34	+
A7301	Lyrm4	LYR motif containing 4	NM_201358	0.0004	-
C1801	Itpripl2	inositol 1,4,5-triphosphate receptor Interacting protein-like 2	NM_001033380	4E-11	+
C2401	Tsn	translin	NM_011650	4E-10	+
C4501	Trip4	thyroid hormone receptor interactor 4	NM_016213	0.0006	-
C7102	Tbrg3	transforming growth factor beta regulated gene 3	NR_027799	0.001	+
G0101	Psme4	proteasome activator subunit 4	NM_014614	4E-87	+
G0501	Gatad2a	GATA zinc finger domain containing 2A	NM_001113346	8E-15	+
G0901	Hoxa10	Homeobox A10 (HOXA10)	NM_008263	1E-47	+
G1101	Iap	Syrian hamster intracisternal A particle	M10134	3E-73	+
G2303	Zc3h12c	zinc finger CCCH type containing 12C	NM_001162921	7E-9	-
G6801	Fst	follistatin	NM_006350	3E-42	-
Ribosomal proteins
A3703	Rpl37a	ribosomal protein L37a	NM_000998	0.001	+
A3901 C3901	Mrpl45	mitochondrial ribosomal protein L45	NM_025927	2E-10 6E-59	-
A3902	Rps21	ribosomal protein S21	NM_001024	7E-14	+
A4001	./.	mitochondrial 12S ribosomal RNA	X84390	0.0	+
G2002	Rpl27a	60S ribosomal protein L27a	NM_000990	5E-18	+
G3504	Rpl10	ribosomal protein L10	NR_026898	4E-132	+
G3601 G3602	Rpl28	ribosomal protein L28	NM_009081	3E-22 8E-180	+
G5701	Rpl22	ribosomal protein L22	NM_000983	0.00009	+

The DEHP effect is identified by (+) for 2.0-fold over-expression, (++) for 10-fold over-expression, (-) for 2.0-fold under-expression and (--) for 10.0-fold under-expression for at least one dose of DEHP. The 37 Differential Display fragments showing no match after comparison with the RefSeq database were not listed in the table. Id (Identification Number) represents the internal reference of the sequence used before the identification of Differential Display sequence.

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