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Table 2 Characteristics of seven novel ORFs

From: A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF

Gene tag Strand Length
(AA)
Overlapsa) Annotation in other enterobacteria
BIO01608b) + 80 No Hypothetical protein
BIO50043b) - 365 Partial (S) Sulfate/thiosulfate transporter subunit
BIO07235b) + 25 Partial (S) None
BIO43803b) - 496 Partial (C) Hypothetical protein
BIO68373 - 59 Nested (C) Conserved hypothetical protein
BIO58539 - 86 Nested (S) None
BIO48527 - 36 Nested (S) None
  1. a) No, ORFs not overlapping other genes; Partial (C), ORFs partially overlapping known genes on the complementary strand; Partial (S), ORFs partially overlapping known genes on the same strand; Nested (C), ORFs completely contained within known genes on the complementary strand; Nested (S), ORFs completely contained within known genes on the same strand, but in a different frame.
  2. b) The transcripts of novel ORFs were confirmed by RT-PCR assay.
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