A proteogenomic analysis of Shigella flexneri using 2D LC-MALDI TOF/TOF

BMC Genomics

Table 2 Characteristics of seven novel ORFs

Gene tag	Strand	Length (AA)	Overlaps^a)	Annotation in other enterobacteria
BIO01608^b)	+	80	No	Hypothetical protein
BIO50043^b)	-	365	Partial (S)	Sulfate/thiosulfate transporter subunit
BIO07235^b)	+	25	Partial (S)	None
BIO43803^b)	-	496	Partial (C)	Hypothetical protein
BIO68373	-	59	Nested (C)	Conserved hypothetical protein
BIO58539	-	86	Nested (S)	None
BIO48527	-	36	Nested (S)	None

a) No, ORFs not overlapping other genes; Partial (C), ORFs partially overlapping known genes on the complementary strand; Partial (S), ORFs partially overlapping known genes on the same strand; Nested (C), ORFs completely contained within known genes on the complementary strand; Nested (S), ORFs completely contained within known genes on the same strand, but in a different frame.
b) The transcripts of novel ORFs were confirmed by RT-PCR assay.

ISSN: 1471-2164