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Table 4 Primers used in real-time PCR amplification of bacteria and host gene.

From: Conjugating effects of symbionts and environmental factors on gene expression in deep-sea hydrothermal vent mussels

Gene

Primer sequence 5'-3'

Sulfide oxidizer symbiont 16S

Forward GAGTAACGCGTAGGAATCTGC

 

Reverse CGAAGGTCCTCCACTTTACTCCATAGAG

Methanotrophic symbiont 16S

Forward GTGCCAGCMGCCGCGGTAA

 

Reverse GCTCCGCCACTAAGCCTATAAATAGACC

Cytosolic malate dehydrogenase (host)

Forward ATGGAGGAAAGAGATATGGCACTGAGCGT

 

Reverse TAACATTAAACATAGCCTAGGAACCTAATG

ATP sulfurylase (SOX)

Forward GTGCGTGATGCCGCTATCCGCACCATG

 

Reverse GGTCCGGCATAGAGCATGTCAAACGGATA

Particulate methane monooxygenase A (MOX)

Forward GAGTGGATTAACAGATATTTGAACTTCTGG

 

Reverse CATACCACCAACAACAGCTGTAAGTACAAA

Ribosomal protein L15 (host)

Forward TATGGTAAACCTAAGACACAAGGAGT

 

Reverse TGGAATGGATCAATCAAAATGATTTC

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BMC Genomics

ISSN: 1471-2164

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