Figure 8

Fluorescent in situ hybridization (FISH) with TRPC-21A-MM short probe. A: bone marrow metaphase plates; B: one of the metaphase sets of chromosomes negative DAPI-banded, numbers of signal bearing chromosomes are indicated. For A and B: DAPI in blue, FISH signal in green; bar - 5 μm. C: all chromosomes karyotyped. In each group the middle image is G-banded mouse chromosome from atlas [41], the side negative DAPI-banded chromosomes are from the plate shown on B. Nine chromosomes with the label are indicated by circles; four chromosomes, with in situ signal that confirmed in silico prediction, are indicated by orange circle. The assembled chromosome 4 has short TRPC-21A-MM array but does not have signal (indicated by empty orange circle). Chromosome 7 has signal in pericentromeric region instead of predicted in silico 7D1 band.