Table 2 Statistics of putative genes involved in camptothecin biosynthesisa
Enzyme code | Name of enzyme | Number of unigenes in cDNA library | Number of ESTs in cDNA library | Number of nucleotides in GenBank |
|---|---|---|---|---|
2.3.1.9 | acetoacetyl-CoA thiolase | 2 | 15 | 0 |
2.3.3.10 | HMG-CoA synthase | 4 | 7 | 1 |
1.1.1.34 | HMG-CoA reductase | 2 | 9 | 3 |
2.7.1.36 | mevalonate kinase | 1 | 5 | 0 |
2.7.4.2 | phosphomevalonate kinase | 2 | 2 | 0 |
4.1.1.33 | mevalonate-5-diphosphate decarboxylase | 2 | 6 | 0 |
2.2.1.7 | DXP synthase | 8 | 105 | 0 |
1.1.1.267 | DXP reductoisomerase | 1 | 19 | 1 |
2.7.7.60 | MEP cytidylyltransferase | 0 | 0 | 0 |
2.7.1.148 | CDP-ME kinase | 3 | 3 | 0 |
4.6.1.12 | MECDP synthase | 1 | 1 | 0 |
1.17.7.1 | 4-hydroxy-3-methylbut-2-enyl-diphosphate synthase | 1 | 33 | 0 |
1.17.1.2 | 4-hydroxy-3-methylbut-2-enyl-diphosphate reductase | 1 | 13 | 0 |
5.3.3.2 | isopentenyl-PP isomerase | 2 | 11 | 1 |
2.5.1.10 | farnesyl diphosphate synthase | 4 | 13 | 0 |
1.14.14.1 | geraniol 10-hydroxylase | 1 | 11 | 0 |
1.-.-.- | 10-HG oxidoreductase | 3 | 84 | 1 |
1.3.3.9 | secologanin synthase | 17 | 165 | 0 |
4.2.1.20 | β-subunit of tryptophan synthase | 4 | 4 | 2 |
4.1.1.28 | tryptophan decarboxylase | 1 | 1 | 2 |
4.3.3.2 | strictosidine synthase | 6 | 14 | 0 |
Total number | 66 | 521 | 11 | |