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Table 3 Assessment of overlapping reliability at end to end merging points by using PCR

From: Generation of the first BAC-based physical map of the common carp genome

Clones overlapping each other

cutoff value of end merging

Positive(+)/Negative(-)

CYC054L01

CYC121J09

1e-40

+

CYC204K23

CYC054L01

1e-40

+

CYC027I24

CYC203H04

8e-37

+

CYC096 P15

CYC148K22

4e-36

+

CYC016K05

CYC207J08

5e-25

+

CYC013P01

CYC240P24

1e-21

+

CYC086L08

CYC118D14

4e-36

+

CYC055K17

CYC098L15

2e-28

+

  1. Note that positive (+) indicates two overlapping clones are truly overlapped at the end-to-end merging point.