Definition of orthologous proteins. Representation of the identity (%) and coverage (%) of the query alignment that results from a BLASTP search of P. acanthamoebae versus L. drancourtii. Of the 1023 proteins showing reciprocal best blast hits, 508 exhibited a similarity greater than 30% and an alignment coverage longer than 60% of the query (black) and were defined as orthologues. Of the remaining 515 proteins (gray), 53 did not fulfill the same criteria in the reciprocal analysis and, therefore were discarded, although they appear on this figure as passing the cutoffs. The correlation coefficient between both of the reciprocal BLAST analyses was 0.97 and 0.80 for the percentage identity and the percentage coverage, respectively.